Depolymerization of phenolic polymers using horseradish peroxidase in organic solvent

被引：15

作者：

Joo, H ^{[1
]}

Chae, HJ ^{[1
]}

Yeo, JS ^{[1
]}

Yoo, YJ ^{[1
]}

机构：

[1] SEOUL NATL UNIV, DEPT CHEM ENGN, SEOUL 151742, SOUTH KOREA

来源：

PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 04期

关键词：

horseradish peroxidase; phenol polymerization; depolymerization; hydrogen peroxide; organic solvent;

D O I：

10.1016/S0032-9592(96)00092-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lignin-like phenolic polymer (polyphenol) which was synthesized by horseradish peroxidase (HRP, EC 1.11.1.7) in a 50% (v/v) dioxane/water mixture was degraded by the same enzyme. The onset of depolymerization was linked to HRP compound-III formation and depended on the specific hydrogen peroxide:HRP ratio at the given phenol concentration. The cut-off value, [H2O2]/[HRP] vs [phenol], required for the depolymerization increased logarithmically as the phenol concentration increased. Compound-III served as a key marker used to analyse reaction performance, especially the onset of depolymerization. (C) 1997 Elsevier Science Ltd.

引用

页码：291 / 296

页数：6

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