Store-operated Ca2+ influx and subplasmalemmal mitochondria

被引:27
作者
Korzeniowski, Marek K. [2 ]
Szanda, Gergoe [1 ]
Balla, Tamas [2 ]
Spaet, Andras [1 ,3 ]
机构
[1] Semmelweis Univ, Dept Physiol, H-1444 Budapest, Hungary
[2] NICHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD USA
[3] Hungarian Acad Sci, Lab Neurobiochem & Mol Physiol, Budapest, Hungary
基金
美国国家卫生研究院;
关键词
Mitochondria; Calcium; Store-operated calcium entry; STIM1; PLASMA-MEMBRANE; CALCIUM-RELEASE; CRAC CHANNEL; CYTOSOLIC CA2+; INSULIN-SECRETION; STIM1; CELLS; ENTRY; ORAI1; ER;
D O I
10.1016/j.ceca.2009.04.002
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Calcium depletion of the endoplasmic reticulum (ER) induces oligomerisation, puncta formation and translocation of the ER Ca2+ sensor proteins, STIM1 and -2 into plasma membrane (PM)-adjacent regions of the ER, where they activate the Orai1, -2 or -3 proteins present in the opposing PM. These proteins form ion channels through which store-operated Ca2+ influx (SOC) occurs. Calcium ions exert negative feedback on SOC. Here we examined whether subplasmalemmal mitochondria, which reduce this feed-back by Ca2+ uptake, are located within or out of the high-Ca2+ microdomains (HCMDs) formed between the ER and plasmalemmal Orai1 channels. For this purpose, COS-7 cells were cotransfected with Orai1, STIM1 labelled with YFP or mRFP and the mitochondrially targeted Ca2+ sensitive fluorescent protein inverse-Pericam. Depletion of ER Ca2+ with ATP + thapsigargin (in Ca2+-free medium) induced the appearance of STIM1 puncta in the <= 100 nm wide subplasmalemmal space, as examined with TIRE Mitochondria were located either in the gaps between STIM1-tagged puncta or in remote, STIM1-free regions. After addition of Ca2+ mitochondrial Ca2+ concentration increased irrespective of the mitochondrion-STIM1 distance. These observations indicate that mitochondria are exposed to Ca2+ diffused laterally from the HCMDs formed between the PM and the subplasmalemmal ER. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:49 / 55
页数:7
相关论文
共 48 条
  • [1] STIM1 converts TRPC1 from a receptor-operated to a store-operated channel: Moving TRPC1 in and out of lipid rafts
    Alicia, Sampieri
    Angelica, Zepeda
    Carlos, Saldana
    Alfonso, Salgado
    Vaca, Luis
    [J]. CELL CALCIUM, 2008, 44 (05) : 479 - 491
  • [2] Calcium microdomains in mitochondria and nucleus
    Alonso, Maria Teresa
    Villalobos, Carlos
    Chamero, Pablo
    Alvarez, Javier
    Garcia-Sancho, Javier
    [J]. CELL CALCIUM, 2006, 40 (5-6) : 513 - 525
  • [3] A guided tour into subcellular colocalization analysis in light microscopy
    Bolte, S.
    Cordelieres, F. P.
    [J]. JOURNAL OF MICROSCOPY, 2006, 224 (213-232) : 213 - 232
  • [4] Mitochondrial Ca2+ uptake depends on the spatial and temporal profile of cytosolic Ca2+ signals
    Collins, TJ
    Lipp, P
    Berridge, MJ
    Bootman, MD
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) : 26411 - 26420
  • [5] A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function
    Feske, S
    Gwack, Y
    Prakriya, M
    Srikanth, S
    Puppel, SH
    Tanasa, B
    Hogan, PG
    Lewis, RS
    Daly, M
    Rao, A
    [J]. NATURE, 2006, 441 (7090) : 179 - 185
  • [6] Energized mitochondria increase the dynamic range over which inositol 1,4,5-trisphosphate activates store-operated calcium influx
    Gilabert, JA
    Bakowski, D
    Parekh, AB
    [J]. EMBO JOURNAL, 2001, 20 (11) : 2672 - 2679
  • [7] Store-operated Ca2+ entry depends on mitochondrial Ca2+ uptake
    Glitsch, MD
    Bakowski, D
    Parekh, AB
    [J]. EMBO JOURNAL, 2002, 21 (24) : 6744 - 6754
  • [8] STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER
    Grigoriev, Ilya
    Gouveia, Susana Montenegro
    Van der Vaart, Babet
    Demmers, Jeroen
    Smyth, Jeremy T.
    Honnappa, Srinivas
    Splinter, Daniel
    Steinmetz, Michel O.
    Putney, James W., Jr.
    Hoogenraad, Casper C.
    Akhmanova, Anna
    [J]. CURRENT BIOLOGY, 2008, 18 (03) : 177 - 182
  • [9] CALCIUM-DEPENDENCE OF THE RATE OF EXOCYTOSIS IN A SYNAPTIC TERMINAL
    HEIDELBERGER, R
    HEINEMANN, C
    NEHER, E
    MATTHEWS, G
    [J]. NATURE, 1994, 371 (6497) : 513 - 515
  • [10] Mitochondrial regulation of store-operated calcium signaling in T lymphocytes
    Hoth, M
    Fanger, CM
    Lewis, RS
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 137 (03) : 633 - 648