T84 epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media

被引：6

作者：

Burleigh, D

Fernandes, K

Perrett, D

机构：

[1] Univ London Queen Mary & Westfield Coll, St Bartholomews & Royal London Sch Med & Dent, Div Biomed Sci, Mol Pharmacol Sect, London E1 4NS, England

[2] St Bartholomews Hosp, Dept Med, London EC1A 7BE, England

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2000年 / 390卷 / 1-2期

关键词：

5-hydroxytryptamine; (5-HT; serotonin); desensitization; serum; T-84; cell;

D O I：

10.1016/S0014-2999(00)00013-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The aim of this study was to establish the cause of insensitivity of T-84 human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T-84 cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T-84 cells gave secretory responses to acetylcholine and forskolin but not to 5-HT. When grown in AIM V serum-free media, T-84 cells responded-to 5-HT. Chromatographic analysis with fluorimetric detection showed high levels of 5-HT (1.8 mu M) in the serum. This contamination is probably responsible for subsequent desensitization of T-84 cells to 5-HT. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：103 / 106

页数：4

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