State of the art in antigen retrieval for immunohistochemistry

被引：93

作者：

D'Amico, Fabio ^{[1
]}

Skarmoutsou, Evangelia ^{[1
]}

Stivala, Franca ^{[1
]}

机构：

[1] Univ Catania, Dept Biomed Sci, I-95124 Catania, Italy

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 341卷 / 1-2期

关键词：

Antigen retrieval; Immunohistochemistry; Fixation; PARAFFIN-EMBEDDED TISSUE; CELL NUCLEAR ANTIGEN; GLUTAMYL-TRANSFERASE TRANSPEPTIDASE; FORMALDEHYDE-INDUCED MODIFICATIONS; TEMPORAL BONE SECTIONS; HUMAN BRAIN-TISSUE; EPOXY SECTIONS; FORMALIN FIXATION; ELECTRON-MICROSCOPY; MONOCLONAL-ANTIBODIES;

D O I：

10.1016/j.jim.2008.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The masking effects of antigens by chemical fixation, processing, embedding media interactions, represent a serious problem for immunohistochemical purposes. Fortunately, different approaches in antigen retrieval exist. These techniques are relatively recent and continuously expanding. This review focuses on the present state of the art in antigen retrieval methods for immunohistochemistry in light and electron microscopy. Moreover, a brief discussion on the chemical aspects of fixation, mechanism of retrieval, as well as its efficacy, is given. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1 / 18

页数：18

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