Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN.

被引:69
作者
Dnyaneshwar, Warude [1 ]
Preeti, Chavan [1 ]
Kalpana, Joshi [1 ]
Bhushan, Patwardhan [1 ]
机构
[1] Univ Pune, Interdisciplinary Sch Hlth Sci, Bioprospecting Lab, Pune 411007, Maharashtra, India
关键词
Phyllanthus emblica; euphorbiaceae; random amplified polymorphic DNA (RAPD); sequence characterized amplified region (SCAR);
D O I
10.1248/bpb.29.2313
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.
引用
收藏
页码:2313 / 2316
页数:4
相关论文
共 21 条
[1]   Gastroprotective effects of 'Amla' Emblica officinalis on in vivo test models in rats [J].
Al-Rehaily, AJ ;
Al-Howiriny, TA ;
Al-Sohaibani, MO ;
Rafatullah, S .
PHYTOMEDICINE, 2002, 9 (06) :515-522
[2]  
[Anonymous], 2002, IND HERB PHARM
[3]  
Bhattacharya Arunabh, 2000, Indian Journal of Experimental Biology, V38, P877
[4]  
Chen KT, 2001, ACTA PHARMACOL SIN, V22, P493
[5]   Generation and characterization of SCARs by cloning and sequencing of RAPD products: A strategy for species-specific marker development in bamboo [J].
Das, M ;
Bhattacharya, S ;
Pal, A .
ANNALS OF BOTANY, 2005, 95 (05) :835-841
[6]   Immunomodulatory effects of agents of plant origin [J].
Ganju, L ;
Karan, D ;
Chanda, S ;
Srivastava, KK ;
Sawhney, RC ;
Selvamurthy, W .
BIOMEDICINE & PHARMACOTHERAPY, 2003, 57 (07) :296-300
[7]  
*GOV IND, 2001, AYURV PHARM IND
[8]   Antitumour activity of Emblica officinalis [J].
Jose, JK ;
Kuttan, G ;
Kuttan, R .
JOURNAL OF ETHNOPHARMACOLOGY, 2001, 75 (2-3) :65-69
[9]  
Joshi K, 2004, CURR SCI INDIA, V87, P159
[10]  
KIRTIKAR KR, 1996, INDIAN MED PLANTS, V3, P2117