Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN.

被引：69

作者：

Dnyaneshwar, Warude ^{[1
]}

Preeti, Chavan ^{[1
]}

Kalpana, Joshi ^{[1
]}

Bhushan, Patwardhan ^{[1
]}

机构：

[1] Univ Pune, Interdisciplinary Sch Hlth Sci, Bioprospecting Lab, Pune 411007, Maharashtra, India

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2006年 / 29卷 / 11期

关键词：

Phyllanthus emblica; euphorbiaceae; random amplified polymorphic DNA (RAPD); sequence characterized amplified region (SCAR);

D O I：

10.1248/bpb.29.2313

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.

引用

页码：2313 / 2316

页数：4

共 21 条

[1] Gastroprotective effects of 'Amla' Emblica officinalis on in vivo test models in rats [J].