Growth and sporulation of Pyrenophora seminiperda in vitro: Effects of culture media, temperature and pH

被引：18

作者：

Campbell, MA ^{[1
]}

Medd, RW ^{[1
]}

Brown, JF ^{[1
]}

机构：

[1] NEW S WALES AGR & FISHERIES,CTR AGR RES & VET,ORANGE,NSW 2800,AUSTRALIA

来源：

MYCOLOGICAL RESEARCH | 1996年 / 100卷

关键词：

D O I：

10.1016/S0953-7562(96)80161-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effects of culture media, temperature and pH on in vitro growth and sporulation of Pyrenophora semeniperda were examined to determine optimal conditions for abundant production of conidia. Radial growth and sporulation (expressed as conidia cm(-2) and conidia colony(-1)) were optimal on modified alphacel culture medium (oatmeal, 10 g; MgSO4.7H(2)O, 1 g; KH2PO4, 1.5 g; NaNo3, 1 g; coconut milk, so ml; agar, 17 g; distilled H2O, 1 l). Growth occurred over the temperature range 5 to less than 35 degrees C and was optimal at 23.2 +/- 0.50. Sporulation occurred over the range 10 to 30 degrees and was optimal at 19.2 +/- 0.05 degrees when expressed as conidia colony(-1), and 18.3 +/- 0.3 degrees when expressed as conidia cm(-2). The pH for maximal growth was 4.7 +/- 0.5. while that for maximal sporulation was 5.4 +/- 0.2 when expressed as conidia colony(-1), and 5.7 +/- 0.1 when expressed as conidia cm(-2).

引用

页码：311 / 317

页数：7

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