Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen

被引:800
作者
Scheible, WR [1 ]
Morcuende, R [1 ]
Czechowski, T [1 ]
Fritz, C [1 ]
Osuna, D [1 ]
Palacios-Rojas, N [1 ]
Schindelasch, D [1 ]
Thimm, O [1 ]
Udvardi, MK [1 ]
Stitt, M [1 ]
机构
[1] Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany
关键词
D O I
10.1104/pp.104.047019
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transcriptome analysis, using Affymetrix ATH1 arrays and a real-time reverse transcription-PCR platform for >1,400 transcription factors, was performed to identify processes affected by long-term nitrogen-deprivation or short-term nitrate nutrition in Arabidopsis. Two days of nitrogen deprivation led to coordinate repression of the majority of the genes assigned to photosynthesis, chlorophyll synthesis, plastid protein synthesis, induction of many genes for secondary metabolism, and reprogramming of mitochondrial electron transport. Nitrate readdition led to rapid, widespread, and coordinated changes. Multiple genes for the uptake and reduction of nitrate, the generation of reducing equivalents, and organic acid skeletons were induced within 30 min, before primary metabolites changed significantly. By 3 h, most genes assigned to amino acid and nucleotide biosynthesis and scavenging were induced, while most genes assigned to amino acid and nucleotide breakdown were repressed. There was coordinate induction of many genes assigned to RNA synthesis and processing and most of the genes assigned to amino acid activation and protein synthesis. Although amino acids involved in central metabolism increased, minor amino acids decreased, providing independent evidence for the activation of protein synthesis. Specific genes encoding expansin and tonoplast intrinsic proteins were induced, indicating activation of cell expansion and growth in response to nitrate nutrition. There were rapid responses in the expression of many genes potentially involved in regulation, including genes for trehalose metabolism and hormone metabolism, protein kinases and phosphatases, receptor kinases, and transcription factors.
引用
收藏
页码:2483 / 2499
页数:17
相关论文
共 53 条
[1]   Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max) [J].
Ranamalie Amarasinghe B.H.R. ;
De Bruxelles G.L. ;
Braddon M. ;
Onyeocha I. ;
Forde B.G. ;
Udvardi M.K. .
Planta, 1998, 206 (1) :44-52
[2]   WAKs: cell wall-associated kinases linking the cytoplasm to the extracellular matrix [J].
Anderson, CM ;
Wagner, TA ;
Perret, M ;
He, ZH ;
He, DZ ;
Kohorn, BD .
PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :197-206
[3]   Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis [J].
Borevitz, JO ;
Xia, YJ ;
Blount, J ;
Dixon, RA ;
Lamb, C .
PLANT CELL, 2000, 12 (12) :2383-2393
[4]   A large family of genes that share homology with CLAVATA3 [J].
Cock, JM ;
McCormick, S .
PLANT PHYSIOLOGY, 2001, 126 (03) :939-942
[5]   NITRATE - NUTRIENT AND SIGNAL FOR PLANT-GROWTH [J].
CRAWFORD, NM .
PLANT CELL, 1995, 7 (07) :859-868
[6]   Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors:: unprecedented sensitivity reveals novel root- and shoot-specific genes [J].
Czechowski, T ;
Bari, RP ;
Stitt, M ;
Scheible, WR ;
Udvardi, MK .
PLANT JOURNAL, 2004, 38 (02) :366-379
[7]   AGRIS:: Arabidopsis Gene Regulatory Information Server, an information resource of Arabidopsis cis-regulatory elements and transcription factors -: art. no. 25 [J].
Davuluri, RV ;
Sun, H ;
Palaniswamy, SK ;
Matthews, N ;
Molina, C ;
Kurtz, M ;
Grotewold, E .
BMC BIOINFORMATICS, 2003, 4 (1)
[8]  
Dill A, 2001, GENETICS, V159, P777
[9]   Establishment of polarity in lateral organs of plants [J].
Eshed, Y ;
Baum, SF ;
Perea, JV ;
Bowman, JL .
CURRENT BIOLOGY, 2001, 11 (16) :1251-1260
[10]   The evolution of CONSTANS-like gene families in barley, rice, and Arabidopsis [J].
Griffiths, S ;
Dunford, RP ;
Coupland, G ;
Laurie, DA .
PLANT PHYSIOLOGY, 2003, 131 (04) :1855-1867