Polymerizable Fab′ antibody fragments for targeting of anticancer drugs

被引：117

作者：

Lu, ZR

Kopecková, P

Kopecek, J ^{[1
]}

机构：

[1] Univ Utah, Dept Pharmaceut & Pharmaceut Chem CCCD, Salt Lake City, UT 84112 USA

[2] Univ Utah, Dept Bioengn, Salt Lake City, UT 84112 USA

来源：

NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 11期

关键词：

polymerizable Fab '; HPMA copolymer; drug targeting; chlorin e(6); anticancer drug;

D O I：

10.1038/15085

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have designed a new pathway for the synthesis of targeted polymeric drug delivery systems, using polymerizable antibody Fab' fragments (MA-Fab'). The targeted systems can be directly prepared by copolymerization of the MA-Fab', N-(2-hydroxypropyl)methacrylamide (HPMA) and drug-containing monomers. Both MA-Fab' and the Fab'-targeted copolymers can effectively bind to target cells. An MA-Fab' (from OV-TL 16 Ab) targeted HPMA copolymer containing mesochlorin e(6) (Mce(6)) was synthesized by copolymerization of MA-Fab', HPMA, and MA-GFLG-Mce(6). The targeted copolymer exhibited a higher cytotoxicity toward OVCAR-3 human ovarian carcinoma cells than the nontargeted Mce(6)-containing copolymer or free Mce(6). The targeted copolymer was internalized more efficiently by OVCAR-3 cells than the nontargeted copolymer.

引用

页码：1101 / 1104

页数：4