Anomalous resonance in a nanomechanical biosensor

被引:127
作者
Gupta, Amit K.
Nair, Pradeep R.
Akin, Demir
Ladisch, Michael R.
Broyles, Steve
Alam, Muhammad A.
Bashir, Rashid [1 ]
机构
[1] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA
[2] Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA
[3] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[4] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA
关键词
nanocantilevers; nanotechnology; protein adsorption; virus detection;
D O I
10.1073/pnas.0602022103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The decrease in resonant frequency (-Delta omega(r)) of a classical cantilever provides a sensitive measure of the mass of entities attached on its surface. This elementary phenomenon has been the basis of a new class of bio-nanomechanical devices as sensing components of integrated microsystems that can perform rapid, sensitive, and selective detection of biological and biochemical entities. Based on classical analysis, there is a widespread perception that smaller sensors are more sensitive (sensitivity approximate to -0.5 omega(r)/m(c), where m(c) is the mass of the cantilever), and this notion has motivated scaling of biosensors to nanoscale dimensions. In this work, we show that the response of a nanomechanical biosensor is far more complex than previously anticipated. Indeed, in contrast to classical microscale sensors, the resonant frequencies of the nanosensor may actually decrease or increase after attachment of protein molecules. We demonstrate theoretically and experimentally that the direction of the frequency change arises from a size-specific modification of diffusion and attachment kinetics of biomolecules on the cantilevers. This work may have broad impact on microscale and nanoscale biosensor design, especially when predicting the characteristics of bio-nanoelectromechanical sensors functionalized with biological capture molecules.
引用
收藏
页码:13362 / 13367
页数:6
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