Transcriptional Landscape of Glomerular Parietal Epithelial Cells

被引：8

作者：

Gharib, Sina A. ^{[1
]}

Pippin, Jeffrey W. ^{[2
]}

Ohse, Takamoto ^{[3
]}

Pickering, Scott G. ^{[2
]}

Krofft, Ronald D. ^{[2
]}

Shankland, Stuart J. ^{[2
]}

机构：

[1] Univ Washington, Ctr Lung Biol, Computat Med Core, Div Pulm & Crit Care Med, Seattle, WA 98195 USA

[2] Univ Washington, Div Nephrol, Seattle, WA 98195 USA

[3] Univ Tokyo, Div Nephrol, Tokyo, Japan

来源：

PLOS ONE | 2014年 / 9卷 / 08期

基金：

美国国家卫生研究院;

关键词：

DE-NOVO EXPRESSION; PODOCYTE PROTEINS; RETINOIC ACID; IN-VITRO; KIDNEY; REGENERATION; PAX8; DIFFERENTIATION; NEPHROPATHY; MICROARRAY;

D O I：

10.1371/journal.pone.0105289

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Very little is known about the function of glomerular parietal epithelial cells (PECs). In this study, we performed genome-wide expression analysis on PEC-enriched capsulated vs. PEC-deprived decapsulated rat glomeruli to determine the transcriptional state of PECs under normal conditions. We identified hundreds of differentially expressed genes that mapped to distinct biologic modules including development, tight junction, ion transport, and metabolic processes. Since developmental programs were highly enriched in PECs, we characterized several of their candidate members at the protein level. Collectively, our findings confirm that PECs are multifaceted cells and help define their diverse functional repertoire.

引用

页数：9

共 39 条

[1]

Recruitment of Podocytes from Glomerular Parietal Epithelial Cells [J].

Appel, Daniel ;

Kershaw, David B. ;

Smeets, Bart ;

Yuan, Gang ;

Fuss, Astrid ;

Frye, Bjoern ;

Elger, Marlies ;

Kriz, Wilhelm ;

Floege, Juergen ;

Moeller, Marcus J. .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2009, 20 (02) :333-343

[2]

Arakawa M, 1977, Contrib Nephrol, V6, P73

[3]

Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[4]

CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[5]

A comparison of normalization methods for high density oligonucleotide array data based on variance and bias [J].

Bolstad, BM ;

Irizarry, RA ;

Åstrand, M ;

Speed, TP .

BIOINFORMATICS, 2003, 19 (02) :185-193

[6]

Nephric lineage specification by Pax2 and Pax8 [J].

Bouchard, M ;

Souabni, A ;

Mandler, M ;

Neubüser, A ;

Busslinger, M .

GENES & DEVELOPMENT, 2002, 16 (22) :2958-2970

[7]

Comparative Evaluation of miRNA Expression between in Vitro and in Vivo Airway Epithelium Demonstrates Widespread Differences [J].

Chen, Peter ;

Edelman, Jeffrey D. ;

Gharib, Sina A. .

AMERICAN JOURNAL OF PATHOLOGY, 2013, 183 (05) :1405-1410

[8]

A METHOD FOR ISOLATION OF MOUSE GLOMERULI FOR QUANTITATION OF IMMUNE DEPOSITS [J].

GAUTHIER, VJ ;

MANNIK, M .

KIDNEY INTERNATIONAL, 1988, 33 (04) :897-899

[9]

Integrative miRNA-mRNA Profiling of Adipose Tissue Unravels Transcriptional Circuits Induced by Sleep Fragmentation [J].

Gharib, Sina A. ;

Khalyfa, Abdelnaby ;

Abdelkarim, Amal ;

Bhushan, Bharat ;

Gozal, David .

PLOS ONE, 2012, 7 (05)

[10]

Emerging roles for retinoids in regeneration and differentiation in normal and disease states [J].

Gudas, Lorraine J. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2012, 1821 (01) :213-221

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