Molecular Characterization of Occult Hepatitis B Cases in Greek Blood Donors

被引：53

作者：

Katsoulidou, Antigoni ^{[1
]}

Paraskevis, Dimitrios ^{[1
]}

Magiorkinis, Emmanouil ^{[1
]}

Moschidis, Zissis ^{[1
]}

Haida, Catherine ^{[1
]}

Hatzitheodorou, Eleni ^{[1
]}

Varaklioti, Agoritsa ^{[2
]}

Karafoulidou, Anastasia ^{[2
]}

Hatzitaki, Maria ^{[3
]}

Kavallierou, Lilian ^{[4
]}

Mouzaki, Athanasia ^{[5
]}

Andrioti, Evaggelia ^{[6
]}

Veneti, Chrysanthi ^{[7
]}

Kaperoni, Athanasia ^{[8
]}

Zervou, Eleftheria ^{[9
]}

Politis, Constantina ^{[10
]}

Hatzakis, Angelos ^{[1
]}

机构：

[1] Univ Athens, Sch Med, Dept Hyg & Epidemiol, GR-11527 Athens, Goudi, Greece

[2] Laikon Gen Hosp, Reg Blood Transfus Ctr 2, Athens, Greece

[3] Blood Donat Ctr Thessaly, Larisa, Greece

[4] A Fleming Blood Transfus Ctr, Athens, Greece

[5] Univ Patras, Sch Med, Dept Internal Med, Div Hematol, GR-26110 Patras, Greece

[6] Hippokrateion Hosp, Athens, Greece

[7] Konstantopoulio Gen Hosp N Ionia, Athens, Greece

[8] Elpis Gen Hosp, Athens, Greece

[9] Univ Hosp, Blood Bank, Ioannina, Greece

[10] Gen Athens Hosp G Gennimatas, Reg Blood Transfus Ctr 3, Athens, Greece

来源：

JOURNAL OF MEDICAL VIROLOGY | 2009年 / 81卷 / 05期

关键词：

blood donors; nucleic acid testing; real-time PCR; sequence analysis; phylogeny; alpha-determinant; SURFACE-ANTIGEN VARIANTS; MULTIPLE SEQUENCE ALIGNMENT; VIRUS-INFECTION; CLINICAL-SIGNIFICANCE; LAMIVUDINE RESISTANCE; FUNCTIONAL-ANALYSIS; DRUG-RESISTANCE; HBV INFECTION; YMDD MOTIF; POLYMERASE;

D O I：

10.1002/jmv.21499

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The use of sensitive nucleic acid testing for hepatitis B virus in blood donors revealed a number of HBV DNA(+) cases among HBsAg(-) donors, a status known as occult HBV infection. The purpose of this study was the serological and molecular characterization of occult HBV infection in Greek blood donors. A prospective study was undertaken in order to identify occult HBV infection cases in blood donors. As part of the routine screening of blood donations in Greece, blood units were screened individually by a multiplex HIV-1/HCV/HBV nucleic acid assay. Initially reactive samples were retested with discriminatory assays. HBV DNA(+)/HBsAg(-) samples were tested further for HBV serological markers and HBV DNA was quantified by real-time PCR. Molecular characterization was performed by sequencing the envelope and polymerase genes of HBV. Preliminary screening revealed 21 occult cases with the following patterns: anti-HBc only: 7 donors, anti-HBc/anti-HBs: 7 donors, anti-HBc/anti-HBe: 5 donors, anti-HBc/anti-HBs/anti-HBe: 2 donors. In all cases, the HBV DNA load was <351 IU/ml. Sequencing was successful in 10 donors (classified within genotype D) revealing several amino acid substitutions related to diagnostic escape and antiviral resistance. HBsAg diagnostic failure and low viral replication in occult HBV infection carriers could possibly be attributed to multiple changes in envelope and polymerase regions, respectively. J. Med. Virol 81:815-825, 2009. (C) 2009 Wiley-Liss, Inc.

引用

页码：815 / 825

页数：11

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