OPTIMIZATION OF METHODOLOGY TO ANALYZE ASCORBIC AND DEHYDROASCORBIC ACID IN VEGETABLES

被引：88

作者：

Campos, Flavia Milagres ^{[1
]}

Rocha Ribeiro, Sonia Machado ^{[1
]}

Della Lucia, Ceres M. ^{[1
]}

Pinheiro-Sant'Ana, Helena Maria ^{[1
]}

Stringheta, Paulo Cesar ^{[2
]}

机构：

[1] Univ Fed Vicosa, Dept Nutr & Saude, BR-36571000 Vicosa, MG, Brazil

[2] Univ Fed Vicosa, Dept Tecnol Alimentos, BR-36571000 Vicosa, MG, Brazil

来源：

QUIMICA NOVA | 2009年 / 32卷 / 01期

关键词：

vitamin C; extraction; chromatography; VITAMIN-C; FRUIT; FOOD; CAROTENOIDS; STORAGE; JUICE;

D O I：

10.1590/S0100-40422009000100017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, different solutions to extract vitamin C were tested. High-performance liquid chromatography was chosen and the conditions were based on isocratic elution in reverse phase column. Dehydroascorbic acid was determined indirectly after its reduction using dithiothreitol. The use of metaphosphoric acid to stabilize the vitamin C was shown to be required and it was necessary to neutralize the pH of the extract to apply dithiothreitol. The average recovery was 90% in collard and tomato samples. The presence of oil did not interfere in extraction and the methodology can be used to analyze stir fried vegetables.

引用

页码：87 / 91

页数：5

共 31 条

[11] Rapid high-performance liquid chromatographic identification/quantification of total vitamin C in fruit drinks [J].

Furusawa, N .

FOOD CONTROL, 2001, 12 (01) :27-29

[12] Kinetic modelling of vitamin C loss in frozen green vegetables under variable storage conditions [J].

Giannakourou, MC ;

Taoukis, PS .

FOOD CHEMISTRY, 2003, 83 (01) :33-41

[13] Enzymatically validated liquid chromatographic method for the determination of ascorbic and dehydroascorbic acids in fruit and vegetables [J].

Gökmen, V ;

Kahraman, N ;

Demir, N ;

Acar, J .

JOURNAL OF CHROMATOGRAPHY A, 2000, 881 (1-2) :309-316

[14] Determination of vitamin C in tropical fruits:: A comparative evaluation of methods [J].

Hernández, Y ;

Lobo, MG ;

González, M .

FOOD CHEMISTRY, 2006, 96 (04) :654-664

[15] Determination of L-ascorbic acid and total ascorbic acid in vascular and nonvascular plants by capillary zone electrophoresis [J].

Herrero-Martínez, JM ;

Simó-Alfonso, E ;

Deltoro, VI ;

Calatayud, A ;

Ramis-Ramos, G .

ANALYTICAL BIOCHEMISTRY, 1998, 265 (02) :275-281

[16] Routine high-performance liquid chromatographic determination of ascorbic acid in foods using L-methionine for the pre-analysis sample stabilization [J].

Iwase, H .

TALANTA, 2003, 60 (05) :1011-1021

[17] Ascorbic acid content of commercial fruit juices and its rate of loss upon storage [J].

Kabasakalis, V ;

Siopidou, D ;

Moshatou, E .

FOOD CHEMISTRY, 2000, 70 (03) :325-328

[18] Improved method for simultaneous determination of ascorbic acid and dehydroascorbic acid, isoascorbic acid and dehydroisoascorbic acid in food and biological samples [J].

Kall, MA ;

Andersen, C .

JOURNAL OF CHROMATOGRAPHY B, 1999, 730 (01) :101-111

[19] STUDY OF MOLAR ABSORPTIVITY OF ASCORBIC-ACID AT DIFFERENT WAVELENGTHS AND PH VALUES [J].

KARAYANNIS, MI ;

SAMIOS, DN ;

GOUSETIS, CP .

ANALYTICA CHIMICA ACTA, 1977, 93 (01) :275-279

[20] Preharvest and postharvest factors influencing vitamin C content of horticultural crops [J].

Lee, SK ;

Kader, AA .

POSTHARVEST BIOLOGY AND TECHNOLOGY, 2000, 20 (03) :207-220

← 1 2 3 4 →