Requirement of CD9 on the egg plasma membrane for fertilization

被引:542
作者
Miyado, K
Yamada, G
Yamada, S
Hasuwa, H
Nakamura, Y
Ryu, F
Suzuki, K
Kosai, K
Inoue, K
Ogura, A
Okabe, M
Mekada, E [1 ]
机构
[1] Kurume Univ, Inst Life Sci, Fukuoka 8390861, Japan
[2] Kurume Univ, Res Ctr Innovat Canc Therapy, Fukuoka 8300011, Japan
[3] Kumamoto Univ, Ctr Anim Resources & Dev, Kumamoto 8600811, Japan
[4] Program Promot Basic Res Act Innovat Biosci, Tokyo 1050001, Japan
[5] St Marys Hosp, Fukuoka 8300047, Japan
[6] Natl Inst Infect Dis, Shinjuku Ku, Tokyo 1628640, Japan
[7] Osaka Univ, Genome Informat Res Ctr, Osaka 5650861, Japan
关键词
D O I
10.1126/science.287.5451.321
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
CD9 is an integral membrane protein associated with integrins and other membrane proteins, Mice lacking CD9 were produced by homologous recombination. Both male and female CD9(-/-) mice were born healthy and grew normally, However, the litter size from CD9(-/-) females was less than 2% of that of the wild type. In vitro fertilization experiments indicated that the cause of this infertility was due to;the failure of sperm-egg fusion. When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion.
引用
收藏
页码:321 / 324
页数:4
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