Amphiphilic Porphyrins for Second Harmonic Generation Imaging

被引：130

作者：

Reeve, James E. ^{[2
]}

Collins, Hazel A. ^{[2
]}

De Mey, Kurt ^{[1
]}

Kohl, Michael M. ^{[3
]}

Thorley, Karl J. ^{[2
]}

Paulsen, Ole ^{[3
]}

Clays, Koen ^{[1
]}

Anderson, Harry L. ^{[2
]}

机构：

[1] Univ Leuven, Dept Chem, B-3001 Louvain, Belgium

[2] Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England

[3] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 08期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

HYPER-RAYLEIGH SCATTERING; NONLINEAR-OPTICAL PROPERTIES; HIGH-FREQUENCY DEMODULATION; MULTIPHOTON FLUORESCENCE; CHROMOPHORES;

D O I：

10.1021/ja8061369

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Amphiphilic donor-acceptor meso-ethynyl porphyrins with polar pyridinium electron-acceptor head groups and hydrophobic dialkyl-aniline electron donors have high molecular hyperpolarizabilities (as measured by hyper-Rayleigh scattering) and high affinities for biological membranes. When bound to water droplets in dodecane, or to the plasma membranes of living cells, they can be used for second harmonic generation (SHG) microscopy; an incident light of wavelength 840 nm generates a strong frequency-doubled signal at 420 nm. Copper(II) and nickel(II) porphyrin complexes give similar SHG signals to those of the free-base porphyrins, while exhibiting no detectable two-photon excited fluorescence.

引用

页码：2758 / +

页数：3

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