nfi, the gene for endonuclease V in Escherichia coli K-12

Endonuclease V is specific for single-stranded DNA or for duplex: DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme, The protein was purified to apparent homogeneity, and from its N-terminal sequence, its gene, nfi, was identified, nfi is immediately downstream of hemE, at kb 4208 (90.4 min) on the current chromosomal map of Escherichia coli K-12. This region was cloned, and plasmid insertion and deletion mutants were used to study its molecular organization. Although nfi is the third of four closely spaced, codirectional genes, it is expressed independently.