AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

被引:30
作者
Song, Maoyong
Jiang, Qinting
Xu, Yan
Liu, Hanxia
Lam, Paul K. S.
O'Toole, Desmond K.
Zhang, Qinghua
Giesy, John P.
Jiang, Guibin
机构
[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem Ecotoxicol, Beijing 100085, Peoples R China
[2] City Univ Hong Kong, Dept Biochem & Chem, Res Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
[3] Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, Ctr Integrat Toxicol, E Lansing, MI 48824 USA
关键词
H4IIE-luc; dioxin; PCBs;
D O I
10.1016/j.chemosphere.2005.08.065
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Total concentrations of compounds that can cause activation of the aryl hydrocarbon receptor (AhR) in extracts of river sediments from various locations in the Haihe and Dagu Rivers, Tianjin, China were determined by use of the in vitro H4IIE-luc cell line. AhR-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a Florisil column into three Fractions. The response of samples was compared to the TCDD standard and expressed as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TCDD-EQs). Significant dioxin-like activity was observed in each sample. The TCDD-EQs in crude extracts of sediments (SCEs), as determined with the bioassay were 2-4 times greater than the sum of the TCDD-EQs of the eluent from fractions separated with a Florisil column. The results also showed that Fractions 2 and 3 contained most of the AhR-mediated activity. The results obtained by using the bioassay were compared with those of previous measurements of the concentrations of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) and the structurally-similar AhR-active polychlorinated biphenyls (PCBs). It was determined that sediments from the Dagu River contained greater concentrations of TCDD-EQ than did sediments from the Haihe River except at Jingangqiao (location R3), which is associated with industrial activities in the adjacent densely populated area. The concentrations of TCDD-EQs, based on the EC20 and the relative potency ranges, of SCEs ranged from 330 to 930 and 1200 to 13 900 pg TCDD-EQ g(-1) dry wt in Haihe and Dagu Rivers, respectively. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1222 / 1230
页数:9
相关论文
共 35 条
[1]   Brominated dioxin-like compounds: in vitro assessment in comparison to classical dioxin-like compounds and other polyaromatic compounds [J].
Behnisch, PA ;
Hosoe, K ;
Sakai, S .
ENVIRONMENT INTERNATIONAL, 2003, 29 (06) :861-877
[2]   Combinatorial bio/chemical analysis of dioxin and dioxin-like compounds in waste recycling, feed/food, humans/wildlife and the environment [J].
Behnisch, PA ;
Hosoe, K ;
Sakai, S .
ENVIRONMENT INTERNATIONAL, 2001, 27 (06) :495-519
[3]   Patterns and levels of organochlorines (DDTs, PCBs, non-ortho PCBs and PCDD/Fs) in male harbour porpoises (Phocoena phocoena) from the Baltic Sea, the Kattegat-Skagerrak Seas and the west coast of Norway [J].
Berggrena, P ;
Ishaq, R ;
Zebühr, Y ;
Näf, C ;
Bandh, C ;
Broman, D .
MARINE POLLUTION BULLETIN, 1999, 38 (12) :1070-1084
[4]  
BROUWER A, 1995, EUR J PHARM-ENVIRON, V293, P1
[5]   The Ah receptor: A regulator of the biochemical and toxicological actions of structurally diverse chemicals [J].
Denison, MS ;
Heath-Pagliuso, S .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1998, 61 (05) :557-568
[6]  
Denison MS, 1998, TARG ORG T, P3
[7]   Cell bioassays for detection of aryl hydrocarbon (AhR) and estrogen receptor (ER) mediated activity in environmental samples [J].
Giesy, JP ;
Hilscherova, K ;
Jones, PD ;
Kannan, K ;
Machala, M .
MARINE POLLUTION BULLETIN, 2002, 45 (1-12) :3-16
[8]   Dioxin-like and non-dioxin-like toxic effects of polychlorinated biphenyls (PCBs): Implications for risk assessment [J].
Giesy, JP ;
Kannan, K .
CRITICAL REVIEWS IN TOXICOLOGY, 1998, 28 (06) :511-569
[9]  
GIESY JP, 1994, ENVIRON SCI TECHNOL, V28, P128
[10]  
GIESY JP, 1994, DIOXIN HLTH, P254