Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar:: expressed sequence tag analysis identifies genes associated with fungal pathogenesis

被引：67

作者：

Li, RG ^{[1
]}

Rimmer, R ^{[1
]}

Buchwaldt, L ^{[1
]}

Sharpe, AG ^{[1
]}

Séguin-Swartz, G ^{[1
]}

Coutu, C ^{[1
]}

Hegedus, DD ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Saskatoon, SK S7N 0X2, Canada

来源：

FUNGAL GENETICS AND BIOLOGY | 2004年 / 41卷 / 08期

关键词：

descriptors; Sclerotinia sclerotiorum; cDNA library; expressed sequence tags; pathogenesis; fungal development; signal transduction;

D O I：

10.1016/j.fgb.2004.03.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Selerotinia sclerotiorum is a ubiquitous necrotrophic fungal pathogen capable of infecting a wide range of plants. To identify genes involved in fungal development and pathogenesis we generated 2232 expressed sequence tags (ESTs) from two cDNA libraries constructed using either mycelia grown in pectin medium or tissues from infected Brassica napus stems. A total of 774 individual fungal genes were identified of which 39 were represented only among the infected plant EST collection. Annotation of 534 unigenes was possible following the categories applied to Saccharomyces cerevisiae and the Universal Gene Ontology scheme. cDNAs were identified that encoded potential pathogenicity factors including four endopolygalacturonases, two exopolygalacturonases, and several metabolite transporters. The potential role of these genes, as well as those encoding signal transduction factors, in the infection process is discussed. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：735 / 753

页数：19

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[1] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].