Characterization of compounds in the Chinese herbal drug Mu-Dan-Pi by liquid chromatography coupled to electrospray ionization mass spectrometry

被引:74
作者
Xu, Shun-jun
Yang, Liu
Zeng, Xing
Zhang, Mian
Wang, Zheng-tao [1 ]
机构
[1] China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210038, Peoples R China
[2] Jinan Univ, Inst Life & Hlth Engn, Guangzhou 510632, Guangdong, Peoples R China
[3] Guangzhou Univ TCM, Affiliated Hosp 2, Guangzhou 510120, Peoples R China
关键词
D O I
10.1002/rcm.2717
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cortex Moutan is a well-known traditional Chinese medicine derived from Paeonia suffruticosa ANDREWS. However, root cortices of P. delavayi and P. decomposita also are used under the name of this drug in some regions such as Yunnan and Sichuan Provinces, respectively. In order to make a comparison of their chemical constituents, the compounds of the three Paeonia species were analyzed by high-performance liquid chromatography-diode array detection/electrospray ionization and quadrupole-time-of-flight tandem mass spectrometry (HPLC-DAD/ESI-MS2). A total of 50 compounds were observed in the 50% (v/v) methanolic extracts, including 17 monoterpenes, 14 galloyl glucoses, 10 acetophenones, 5 phenolic acids, 3 flavonoids and 1 triterpene. These chemical constituents were separated on a C-18 column and identified or tentatively characterized based on UV spectra and MS fragmentation behavior. The chemical compositions of the three Paeonia species were found to have many differences. Paeonol was the predominant constituent of P. suffruticosa and P. decomposita, while P. delavayi contained albiflorin and more galloyl glucoses than the other two Paeonia species. Most of these identified compounds have been reported from P. delavayi and P. decomposita for the first time. The ESI-MS fragmentation behavior of monoterpene glycosides, acetophenones and galloyl glucoses was also investigated successively, and appropriate characteristic pathways were proposed. The large differences in chemical compounds among the three Paeonia species strongly encouraged further comparison of the bioactivities of these three species. Copyright (c) 2006 John Wiley & Sons, Ltd.
引用
收藏
页码:3275 / 3288
页数:14
相关论文
共 23 条
[1]   A capsule review of recent studies on the application of mass spectrometry in the analysis of Chinese medicinal herbs [J].
Cai, ZW ;
Lee, FSC ;
Wang, XR ;
Yu, WJ .
JOURNAL OF MASS SPECTROMETRY, 2002, 37 (10) :1013-1024
[2]  
CONG P, 2000, ANAL CHEM HDB MASS S, P307
[3]  
Ding HY, 1999, CHEM PHARM BULL, V47, P652
[4]   Phytochemical and pharmacological studies on Chinese Paeonia species [J].
Ding, HY ;
Lin, HC ;
Teng, CM ;
Wu, YC .
JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 2000, 47 (02) :381-388
[5]  
ERLEND H, 2002, RAPID COMMUN MASS SP, V16, P655
[6]  
FABRE N, 2001, J AM SOC MASS SPECTR, V36, P169
[7]   PHARMACOLOGICAL STUDIES ON ROOT BARK OF PAEONIA MOUTAN .I. CENTRAL EFFECTS OF PAEONOL [J].
HARADA, M ;
YAMASHITA, A .
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1969, 89 (09) :1205-+
[8]   PAEONILACTONE-A, PAEONILACTONE-B, AND PAEONILACTONE-C, NEW MONOTERPENOIDS FROM PAEONY ROOT [J].
HAYASHI, T ;
SHINBO, T ;
SHIMIZU, M ;
ARISAWA, M ;
MORITA, N ;
KIMURA, M ;
MATSUDA, S ;
KIKUCHI, T .
TETRAHEDRON LETTERS, 1985, 26 (31) :3699-3702
[9]   On-line identification of phytochemical constituents in botanical extracts by combined high-performance liquid chromatographic-diode array detection-mass spectrometric techniques [J].
He, XG .
JOURNAL OF CHROMATOGRAPHY A, 2000, 880 (1-2) :203-232
[10]  
LANG HY, 1990, TETRAHEDRON, V46, P3123