Elucidation of carotenoid patterns in citrus products by means of comprehensive normal-phase x reversed-phase liquid chromatography

被引:88
作者
Dugo, Paola
Skerikova, Veronika
Kumm, Tiina
Trozzi, Alessandra
Jandera, Pavel
Mondello, Luigi
机构
[1] Univ Messina, Fac Sci MM FF NN, Dipartimento Sci Alimenti & Ambiente, I-98166 Messina, Italy
[2] Univ Messina, Fac Farm, Dipartimento Farmacochim, I-98168 Messina, Italy
[3] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice 53210, Czech Republic
[4] Univ Messina, Fac Farm, Dipartimento Farmacobiol, I-98168 Messina, Italy
关键词
MASS-SPECTROMETRIC DETECTION; VALENCIA ORANGE JUICE; DIODE-ARRAY DETECTION; GEOMETRICAL-ISOMERS; FOOD ANALYSIS; SWEET ORANGE; IDENTIFICATION; SEPARATION; QUANTIFICATION; PIGMENTS;
D O I
10.1021/ac061290q
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel approach for carotenoid analysis has been developed. Orange essential oil and juice carotenoids were separated by means of comprehensive dual-gradient elution HPLC, using normal phase with a microbore silica column in the first dimension ( first D), reversed phase with a monolithic C18 column in the second dimension ( second D), and a 10-port switching valve as an interface. An on-line photodiode array detector was used in order to obtain absorption spectra. Peak identification was obtained by combining retention data with the UV-visible spectra.
引用
收藏
页码:7743 / 7750
页数:8
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