Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers

被引：27

作者：

Hernandez, Frank Jeyson ^{[3
]}

Dondapati, Srujan Kumar ^{[4
,5
]}

Ozalp, V. Cengiz ^{[3
]}

Pinto, Alessandro ^{[3
]}

O'Sullivan, Ciara K. ^{[3
]}

Klar, Thomas A. ^{[1
,2
]}

Katakis, Ioannis ^{[3
]}

机构：

[1] Tech Univ Ilmenau, Inst Phys, D-98684 Ilmenau, Germany

[2] Tech Univ Ilmenau, Inst Micro & Nanotechnol, D-98684 Ilmenau, Germany

[3] Univ Rovira & Virgili, Dept Chem Engn, Bioengn & Bioelectrochem Grp, Tarragona 43007, Spain

[4] Univ Munich, Dept Phys, Photon & Optoelect Grp, D-80799 Munich, Germany

[5] Univ Munich, CeNS, D-80799 Munich, Germany

来源：

JOURNAL OF BIOPHOTONICS | 2009年 / 2卷 / 04期

关键词：

apatamer; gold nanoparticles; biosensing; SURFACE-PLASMON RESONANCE; PROTEIN BIOSENSOR; RNA; MOLECULES; SELECTION; DNA; LIGANDS; INVITRO; BINDING;

D O I：

10.1002/jbio.200910006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta-nano-sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta-sensor may be used in nanoscopic volumes such as in array type assays or even inside cells.

引用

页码：227 / 231

页数：5

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