Separation and detection of closely related peptides by micellar electrokinetic chromatography coupled with electrospray ionization mass spectrometry using the partial filling technique

被引:69
作者
Koezuka, K
Ozaki, H
Matsubara, N
Terabe, S
机构
[1] HIMEJI INST TECHNOL,FAC SCI,KAMIGORI,HYOGO 67812,JAPAN
[2] KANEKA TECHNO RES CO LTD,HYOGO KU,KOBE,HYOGO 652,JAPAN
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 689卷 / 01期
关键词
partial filling method; peptides; neurotensin; angiotensin; sucrose monododecanoate;
D O I
10.1016/S0378-4347(96)00370-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Closely related peptides such as neurotensin and angiotensin analogues were separated by capillary zone electrophoresis using a nonionic surfactant, sucrose monododecanoate, as a micelle forming reagent. These peptides were detected by an on-line coupled mass spectrometer using an electrospray ionization interface. However, the presence of the micelles in the separation solution drastically reduced the sensitivity of the mass spectrometer. Therefore, a partial filling technique was employed to prevent the micelles from entering the mass spectrometric interface. A part of the capillary from the injection end was filled with the micellar solution in this technique. Analytes passed through the micellar zone during the electrophoresis and when the separated analytes reached the detection end of the capillary, the micellar zone was still behind the analyte zones, because the nonionic surfactant moved very slowly in acidic conditions. Thus the technique was very useful for mass spectrometric detection for CE when the micellar solution was employed for separation. The optimization of separation and detection conditions was investigated.
引用
收藏
页码:3 / 11
页数:9
相关论文
共 22 条
[1]  
[Anonymous], RAPID COMMUN MASS SP
[2]   COUPLING CAPILLARY ZONE ELECTROPHORESIS AND CONTINUOUS-FLOW FAST ATOM BOMBARDMENT MASS-SPECTROMETRY FOR THE ANALYSIS OF PEPTIDE MIXTURES [J].
CAPRIOLI, RM ;
MOORE, WT ;
MARTIN, M ;
DAGUE, BB ;
WILSON, K ;
MORING, S .
JOURNAL OF CHROMATOGRAPHY, 1989, 480 :247-257
[3]   ONLINE COUPLING OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY TO ELECTROSPRAY MASS-SPECTROMETRY [J].
LAMOREE, MH ;
TJADEN, UR ;
VANDERGREEF, J .
JOURNAL OF CHROMATOGRAPHY A, 1995, 712 (01) :219-225
[4]   FRACTION COLLECTION AFTER AN OPTIMIZED MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHIC-SEPARATION OF NUCLEIC-ACID CONSTITUENTS [J].
LECOQ, AF ;
DIBIASE, S ;
MONTANARELLA, L .
JOURNAL OF CHROMATOGRAPHY, 1993, 638 (02) :363-373
[5]   ONLINE CAPILLARY ZONE ELECTROPHORESIS ION SPRAY TANDEM MASS-SPECTROMETRY FOR THE DETERMINATION OF DYNORPHINS [J].
LEE, ED ;
MUCK, W ;
HENION, JD ;
COVEY, TR .
JOURNAL OF CHROMATOGRAPHY, 1988, 458 :313-321
[6]   SEPARATION OF 11 ANGIOTENSIN-II ANALOGS BY CAPILLARY ELECTROPHORESIS WITH A NONIONIC SURFACTANT IN ACIDIC MEDIA [J].
MATSUBARA, N ;
KOEZUKA, K ;
TERABE, S .
ELECTROPHORESIS, 1995, 16 (04) :580-583
[7]   SEPARATION OF CLOSELY RELATED PEPTIDES BY CAPILLARY ELECTROPHORESIS WITH A NONIONIC SURFACTANT [J].
MATSUBARA, N ;
TERABE, S .
CHROMATOGRAPHIA, 1992, 34 (9-10) :493-496
[8]   COUPLING OF CAPILLARY ZONE ELECTROPHORESIS AND CAPILLARY LIQUID-CHROMATOGRAPHY WITH COAXIAL CONTINUOUS-FLOW FAST ATOM BOMBARDMENT TANDEM SECTOR MASS-SPECTROMETRY [J].
MOSELEY, MA ;
DETERDING, LJ ;
TOMER, KB ;
JORGENSON, JW .
JOURNAL OF CHROMATOGRAPHY, 1989, 480 :197-209
[9]   CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY [J].
NIESSEN, WMA ;
TJADEN, UR ;
VANDERGREEF, J .
JOURNAL OF CHROMATOGRAPHY, 1993, 636 (01) :3-19
[10]   MICELLAR ELECTROKINETIC CHROMATOGRAPHY MASS-SPECTROMETRY USING A HIGH-MOLECULAR-MASS SURFACTANT - ONLINE COUPLING WITH AN ELECTROSPRAY-IONIZATION INTERFACE [J].
OZAKI, H ;
ITOU, N ;
TERABE, S ;
TAKADA, Y ;
SAKAIRI, M ;
KOIZUMI, H .
JOURNAL OF CHROMATOGRAPHY A, 1995, 716 (1-2) :69-79