ONLINE COUPLING OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY TO ELECTROSPRAY MASS-SPECTROMETRY

被引:44
作者
LAMOREE, MH [1 ]
TJADEN, UR [1 ]
VANDERGREEF, J [1 ]
机构
[1] LEIDEN UNIV,LEIDEN AMSTERDAM CTR DRUG RES,DIV ANALYT CHEM,2300 RA LEIDEN,NETHERLANDS
关键词
D O I
10.1016/0021-9673(95)00507-J
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The possibility of selectivity enhancement in capillary electrophoresis-mass spectrometry (CE-MS) by hyphenating micellar electrokinetic chromatography (MEKC) and electrospray mass spectrometry (MS) is described for two quaternary ammonium compounds. Direct coupling of MEKC to MS is hazardous because of the contamination of the ion source due to presence of an excess of micelle forming agent in the MEKC buffer. Therefore, a coupled-capillary setup with the possibilities of voltage switching and buffer renewal has been designed. Such a system allows on-line heartcutting of the zones of interest in the MEKC capillary with subsequent transfer via a second capillary to the mass spectrometer.
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页码:219 / 225
页数:7
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