APPLICATION OF ELECTROKINETIC CHROMATOGRAPHY TO PHARMACEUTICAL ANALYSIS

被引:31
作者
NISHI, H [1 ]
TERABE, S [1 ]
机构
[1] HIMEJI INST TECHNOL,FAC SCI,KAMIGORI,HYOGO 67812,JAPAN
关键词
MICELLAR ELECTROKINETIC CHROMATOGRAPHY; SELECTIVITY MANIPULATION; DIRECT INJECTION METHOD; CHIRAL SEPARATION;
D O I
10.1016/0731-7085(93)80114-G
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Electrokinetic chromatography (EKC) is one branch of capillary electrophoresis that permits the separation of electrically neutral solutes by the electrophoretic technique. The separation principle of EKC is based on that of chromatography, and various modes of EKC have been developed along with the partition mechanism. Micellar electrokinetic chromatography (MEKC), in which an ionic surfactant solution is employed instead of a buffer solution in capillary zone electrophoresis (CZE) at a higher concentration than the critical micelle concentration (CMC), has become the most popular technique among various EKC modes. Besides the separation of the electrically neutral or non-ionic solutes, MEKC is also effective for the separation of ionic solutes as well as CZE, hence it suits the analysis of pharmaceuticals, including cationic, anionic and neutral. Separation selectivity in MEKC can be manipulated easily through the modification of the buffer as well as changing the surfactants. Direct enantiomeric separation is successful by using chiral surfactants or chiral additives. Determination of drugs in plasma is also successfully achieved by a direct sample injection method, similar to micellar HPLC. In this paper, we summarize the principle separation characteristics of MEKC and some applications to pharmaceutical analysis, including direct enantiomeric separation and direct assay of drugs in plasma.
引用
收藏
页码:1277 / 1287
页数:11
相关论文
共 55 条