POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

被引：63

作者：

Lausted, C ^{[1
]}

Dahl, T ^{[1
]}

Warren, C ^{[1
]}

King, K ^{[1
]}

Smith, K ^{[1
]}

Johnson, M ^{[1
]}

Saleem, R ^{[1
]}

Aitchison, J ^{[1
]}

Hood, L ^{[1
]}

Lasky, SR ^{[1
]}

机构：

[1] Inst Syst Biol, Seattle, WA 98103 USA

来源：

GENOME BIOLOGY | 2004年 / 5卷 / 08期

关键词：

D O I：

10.1186/gb-2004-5-8-r58

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.

引用

页数：17

共 31 条

[1] High-density oligonucleotide arrays [J].

Blanchard, AP ;

Kaiser, RJ ;

Hood, LE .

BIOSENSORS & BIOELECTRONICS, 1996, 11 (6-7) :687-690

[2] Minimum information about a microarray experiment (MIAME) - toward standards for microarray data [J].

Brazma, A ;

Hingamp, P ;

Quackenbush, J ;

Sherlock, G ;

Spellman, P ;

Stoeckert, C ;

Aach, J ;

Ansorge, W ;

Ball, CA ;

Causton, HC ;

Gaasterland, T ;

Glenisson, P ;

Holstege, FCP ;

Kim, IF ;

Markowitz, V ;

Matese, JC ;

Parkinson, H ;

Robinson, A ;

Sarkans, U ;

Schulze-Kremer, S ;

Stewart, J ;

Taylor, R ;

Vilo, J ;

Vingron, M .

NATURE GENETICS, 2001, 29 (04) :365-371

[3] Quantifying DNA-protein interactions by double-stranded DNA arrays [J].

Bulyk, ML ;

Gentalen, E ;

Lockhart, DJ ;

Church, GM .

NATURE BIOTECHNOLOGY, 1999, 17 (06) :573-577

[4] Making and reading microarrays [J].

Cheung, VG ;

Morley, M ;

Aguilar, F ;

Massimi, A ;

Kucherlapati, R ;

Childs, G .

NATURE GENETICS, 1999, 21 (Suppl 1) :15-19

[5]

DERISI J, 1998, MGUIDE COMPLETE GUID

[6]

Derisi Joseph L., 1999, Current Opinion in Oncology, V11, P76, DOI 10.1097/00001622-199901000-00015

[7] OPTICAL MELTING OF 128-OCTAMER DNA DUPLEXES - EFFECTS OF BASE-PAIR LOCATION AND NEAREST NEIGHBORS ON THERMAL-STABILITY [J].

DOKTYCZ, MJ ;

MORRIS, MD ;

DORMADY, SJ ;

BEATTIE, KL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8439-8445

[8] A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids [J].

Gao, XL ;

LeProust, E ;

Zhang, H ;

Srivannavit, O ;

Gulari, E ;

Yu, PL ;

Nishiguchi, C ;

Xiang, Q ;

Zhou, XC .

NUCLEIC ACIDS RESEARCH, 2001, 29 (22) :4744-4750

[9]

HORVATH SJ, 1987, METHOD ENZYMOL, V154, P314

[10] Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer [J].

Hughes, TR ;

Mao, M ;

Jones, AR ;

Burchard, J ;

Marton, MJ ;

Shannon, KW ;

Lefkowitz, SM ;

Ziman, M ;

Schelter, JM ;

Meyer, MR ;

Kobayashi, S ;

Davis, C ;

Dai, HY ;

He, YDD ;

Stephaniants, SB ;

Cavet, G ;

Walker, WL ;

West, A ;

Coffey, E ;

Shoemaker, DD ;

Stoughton, R ;

Blanchard, AP ;

Friend, SH ;

Linsley, PS .

NATURE BIOTECHNOLOGY, 2001, 19 (04) :342-347

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