Effect of NaCl, glutathione and ascorbic acid on function of tonoplast vesicles isolated from barley leaves

被引：18

作者：

Chen, Q ^{[1
]}

Zhang, WH ^{[1
]}

Liu, YL ^{[1
]}

机构：

[1] Nanjing Agr Univ, Dept Agron, Nanjing 210095, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1999年 / 155卷 / 06期

关键词：

AsA; GSH; Hordeum vulgare L; Tonoplast H+-ATPase; Tonoplast H+-PPase; salt stress;

D O I：

10.1016/S0176-1617(99)80083-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The seedlings of two barley cultivars differing in salt sensitivity were treated with 300 mmol/L NaCl for 0, 2, 4, 6, and 8 days. The activities of H+-ATPase (EC 3.6.1.35) and H+-PPase (EC 3.6.1.1) associated with tonoplast vesicles isolated from leaves were assayed. H+-ATPase activity increased in the salt-tolerant cultivar during the first 4 days of NaCl stress, and decreased later. In the salt-sensitive cultivar, however, H+-ATPase activity decreased after NaCl treatment. Both GSH content and APX activity of barley seedling leaves in the two cultivars showed the same trends as H+-ATPase activity. There was a significantly positive correlation between H+-ATPase activity and APX activity and between H+-ATPase activity and GSH content, respectively Under salt stress, exogenous GSH or AsA stimulated the activities of H+-ATPase and H+-PPase in two cultivars, and diminished MDA content and electrolyte leakage in leaves of two cultivars.

引用

页码：685 / 690

页数：6

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