Transcriptomic responses of Caco-2 cells to Lactobacillus rhamnosus GG and Lactobacillus plantarum J26 against oxidative stress

被引:32
作者
Hou, Yichao [1 ]
Li, Xuesong [1 ]
Liu, Xinyu [1 ]
Zhang, Yashuo [1 ]
Zhang, Wei [1 ]
Man, Chaoxin [1 ]
Jiang, Yujun [1 ]
机构
[1] Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Lactobacillus rhamnosus GG; Lactobacillus plantarum J26; digital gene expression profiling; antioxidant mechanisms; LACTIC-ACID BACTERIA; PULP STEM-CELLS; GENE-EXPRESSION; ANTIOXIDANT ACTIVITY; OSTEOGENIC DIFFERENTIATION; SIGNALING PATHWAY; INNATE IMMUNITY; ACTIVATION; INACTIVATION; EXPOSURE;
D O I
10.3168/jds.2019-16332
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Oxidative stress is the basic reason for aging and age-related diseases. In this study, we investigated the protective effect of 2 strains of lactic acid bacteria (LAB), Lactobacillus rhamnosus GG and L. plantarum J26, against oxidative stress in Caco-2 cells, and gave an overview of the mechanisms of lactic acid bacteria antioxidant activity using digital gene expression profiling. The 2 LAB strains provided significant protection against hydrogen peroxide (H2O2)-induced reduction in superoxide dismutase activity and increase in glutathione peroxidase activity in Caco-2 cells. However, inactive bacteria had little effect on alleviating oxidation stress in Caco-2 cells. Eight genes related to oxidative stress-FOSB, TNF, PPP1R15A, NUAK2, ATF3, TNFAIP3, EGR2, and FBN2-were significantly upregulated in H2O2-induced Caco-2 cells compared with untreated Caco-2 cells. After incubation of the H2O2-induced Caco-2 cells with L. rhamnosus GG and L. plantarum J26, 5 genes (TNF, EGR2, NUAK2, FBN2, and TNFAIP3) and 2 genes (NUAK2 and FBN2) were downregulated, respectively. In addition, the Kyoto Encyclopedia of Genes and Genomes indicated that some signaling pathways associated with inflammation, immune response, and apoptosis, such as Janus kinase/signal transducers and activators of transcription (JakSTAT), mitogen-activated protein kinase (MAPK), nuclear factor-kappa B, and tumor necrosis factor, were all negatively modulated by the 2 strains, especially L. rhamnosus GG. In this paper, we reveal the mechanism of LAB in relieving oxidative stress and provide a theoretical basis for the rapid screening and evaluation of new LAB resources.
引用
收藏
页码:7684 / 7696
页数:13
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