Reliability of Pseudomonas aeruginosa semi-automated rep-PCR genotyping in various epidemiological situations

被引：43

作者：

Doleans-Jordheim, A. ^{[1
,2
,3
,4
]}

Cournoyer, B. ^{[2
,7
]}

Bergeron, E. ^{[2
,3
,4
]}

Croize, J. ^{[5
]}

Salord, H. ^{[6
]}

Andre, J. ^{[2
,3
,4
]}

Mazoyer, M-A. ^{[2
,3
,4
]}

Renaud, F. N. R. ^{[2
,4
]}

Freney, J. ^{[2
,3
,4
]}

机构：

[1] ISPB, Microbiol Lab, F-69373 Lyon 08, France

[2] Univ Lyon, Lyon, France

[3] Univ Lyon 1, ISPB, F-69365 Lyon, France

[4] Microbiol Lab, URTI2B, Lyon, France

[5] Hop A Michalon, Microbiol Lab, Grenoble, France

[6] Hosp Civils Lyon, Microbiol Lab, Hop Croix Rousse, Lyon, France

[7] Univ Lyon 1, Res Grp Bacterial Opportunist Pathogens & Environ, Ecol Microbienne UMR5557, CNRS, F-69622 Villeurbanne, France

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2009年 / 28卷 / 09期

关键词：

FIELD GEL-ELECTROPHORESIS; SEQUENCE-BASED PCR; SYSTEM;

D O I：

10.1007/s10096-009-0755-z

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The purpose of this study was to evaluate the possibility of using a semi-automated repetitive DNA sequences-based polymerase chain reaction (rep-PCR) for typing Pseudomonas aeruginosa isolates. rep-PCR profiles obtained by the DiversiLabA (R) system of 84 P. aeruginosa isolates from distinct epidemiological situations were obtained. rep-PCR groupings were in good agreement with the origin of these isolates. Linked rep-PCR profiles were observed for isolates recovered from a same family of cystic fibrosis (CF) patients, for the etiological agents of clustered cases of nosocomial infections, and for some isolates recovered from a same hospital room. rep-PCR and pulsed-field gel electrophoresis SpeI restricted genomic DNA (PFGE-SpeI) profiles were compared. In a few instances, rep-PCR revealed genetic divergences among isolates of a same group of PFGE-SpeI profiles. These divergences could reflect genetic drifts among closely related isolates, as illustrated by those observed between clinical and environmental isolates of a same group of PFGE-SpeI profiles. The interpretation of such differences will require further studies, but the rep-PCR analysis of P. aeruginosa diversity appeared to be an appropriate method to investigate infra-specific genetic relatedness.

引用

页码：1105 / 1111

页数：7

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