The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals

被引：105

作者：

Kubickova, S ^{[1
]}

Cernohorska, H ^{[1
]}

Musilova, P ^{[1
]}

Rubes, J ^{[1
]}

机构：

[1] Vet Res Inst, CS-62132 Brno, Czech Republic

来源：

CHROMOSOME RESEARCH | 2002年 / 10卷 / 07期

关键词：

chromosome painting; clinical cytogenetics; farm animals; laser microdissection;

D O I：

10.1023/A:1020914702767

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specific painting probes. Its main advantage is the fast non-contact collection of chromosomes.

引用

收藏

页码：571 / 577

页数：7

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