Nisin and lacticin 481 coproduction by Lactococcus lactis strains isolated from raw ewes' milk

被引:33
作者
Bravo, D. [1 ]
Rodriguez, E. [1 ]
Medina, M. [1 ]
机构
[1] INIA, Inst Nacl Invest & Tecnol Agr & Alimentaria, Dept Tecnol Alimentos, Madrid 28040, Spain
关键词
lactococcus; nisin; lacticin; 481; raw ewes' milk; ACID BACTERIA; GENETIC-CHARACTERIZATION; IDENTIFICATION; ANTIMICROBIALS; LANTIBIOTICS; DIVERSITY; CHEESE;
D O I
10.3168/jds.2009-2237
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Bacteriocin-producing lactococci were isolated from raw ewes' milk samples obtained from 5 different Protected Designation of Origin Zamorano cheese manufacturers. Thirteen isolates with antimicrobial activity against Lactococcus lactis HP were selected. Eleven were identified by a PCR technique as L. lactis ssp. lactis and 2 were identified as L. lactis ssp. cremoris. They were grouped under 4 different pulsed-field gel electrophoresis patterns. The presence of structural genes of both nisin and lacticin 481 was detected in 10 L. lactis ssp. lactis isolates belonging to 2 different pulsed-field gel electrophoresis patterns. Coproduction of nisin and lacticin 481 was confirmed after semipurification by using selective indicators. The production of 2 bacteriocins by the same strain is an uncommon property, with relevance in food safety. Nisin and lacticin 481 L. lactis-producing strains might be used as adjunct cultures to the commercial starter in the manufacture of dairy products.
引用
收藏
页码:4805 / 4811
页数:7
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