Uncoupling brassinosteroid levels and de-etiolation in pea

被引:37
作者
Symons, GM [1 ]
Schultz, L [1 ]
Kerckhoffs, LHJ [1 ]
Davies, NW [1 ]
Gregory, D [1 ]
Reid, JB [1 ]
机构
[1] Univ Tasmania, Sch Plant Sci, Hobart, Tas 7001, Australia
关键词
D O I
10.1034/j.1399-3054.2002.1150219.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The suggestion that brassinosteroids (BRs) have a negative regulatory role in de-etiolation is based largely on correlative evidence, which includes the de-etiolated phenotypes of and increased expression of light-regulated genes in, dark-grown mutants defective in BR biosynthesis or response. However, we have obtained the first direct evidence which shows that endogenous BR levels in light-grown pea seedlings are increased, not decreased, in comparison with those grown in the dark. Similarly, we found no evidence of a decrease in castasterone (CS) levels in seedlings that were transferred from the dark to the light for 24 h. Furthermore, CS levels in the constitutively de-etiolated lip1 mutant are similar to those in wild-type plants, and are not reduced as is the case in the BR-deficient 10 plants. Unlike lip1, the pea BR-deficient mutants lk and lkb are not de-etiolated at the morphological or molecular level, as they exhibit neither a de-etiolated phenotype or altered expression of light-regulated genes when grown in the dark. Similarly, dark-grown WT plants treated with the BR biosynthesis inhibitor, Brz, do not exhibit a de-etiolated phenotype. In addition, analysis of the lip1lkb double mutant revealed an additive phenotype indicative of the two genes acting in independent pathways. Together these results strongly suggest that BR levels do not play a negative-regulatory role in de-etiolation in pea.
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页码:311 / 319
页数:9
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共 52 条
  • [1] Regulation of gibberellin 20-oxidase and gibberellin 3β-hydroxylase transcript accumulation during de-etiolation of pea seedlings
    Ait-Ali, T
    Frances, S
    Weller, JL
    Reid, JB
    Kendrick, RE
    Kamiya, Y
    [J]. PLANT PHYSIOLOGY, 1999, 121 (03) : 783 - 791
  • [2] Molecular physiology of brassinosteroids revealed by the analysis of mutants
    Altmann, T
    [J]. PLANTA, 1999, 208 (01) : 1 - 11
  • [3] Recent advances in brassinosteroid molecular genetics
    Altmann, T
    [J]. CURRENT OPINION IN PLANT BIOLOGY, 1998, 1 (05) : 378 - 383
  • [4] Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor
    Asami, T
    Min, YK
    Nagata, N
    Yamagishi, K
    Takatsuto, S
    Fujioka, S
    Murofushi, N
    Yamaguchi, I
    Yoshida, S
    [J]. PLANT PHYSIOLOGY, 2000, 123 (01) : 93 - 99
  • [5] An Arabidopsis brassinosteroid-dependent mutant is blocked in cell elongation
    Azpiroz, R
    Wu, YW
    LoCascio, JC
    Feldmann, KA
    [J]. PLANT CELL, 1998, 10 (02) : 219 - 230
  • [6] GENETIC-ANALYSIS OF THE ROLE OF GIBBERELLIN IN THE RED-LIGHT INHIBITION OF STEM ELONGATION IN ETIOLATED SEEDLINGS
    BEHRINGER, FJ
    DAVIES, PJ
    REID, JB
    [J]. PLANT PHYSIOLOGY, 1990, 94 (02) : 432 - 439
  • [7] Plants steroid hormones, brassinosteroids: Current highlights of molecular aspects on their synthesis/metabolism, transport, perception and response
    Bishop, GJ
    Yokota, T
    [J]. PLANT AND CELL PHYSIOLOGY, 2001, 42 (02) : 114 - 120
  • [8] The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis
    Bishop, GJ
    Nomura, T
    Yokota, T
    Harrison, K
    Noguchi, T
    Fujioka, S
    Takatsuto, S
    Jones, JDG
    Kamiya, Y
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (04) : 1761 - 1766
  • [9] Lesions in the sterol Δ7 reductase gene of Arabidopsis cause dwarfism due to a block in brassinosteroid biosynthesis
    Choe, S
    Tanaka, A
    Noguchi, T
    Fujioka, S
    Takatsuto, S
    Ross, AS
    Tax, FE
    Yoshida, S
    Feldman, KA
    [J]. PLANT JOURNAL, 2000, 21 (05) : 431 - 443
  • [10] The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis
    Choe, S
    Dilkes, BP
    Gregory, BD
    Ross, AS
    Yuan, H
    Noguchi, T
    Fujioka, S
    Takatsuto, S
    Tanaka, A
    Yoshida, S
    Tax, FE
    Feldmann, KA
    [J]. PLANT PHYSIOLOGY, 1999, 119 (03) : 897 - 907