Effects of a heavy-atom salt on the solid-matrix luminescence properties of the (+/-)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[alpha]pyrene-DNA adducts and tetrol I-1 adsorbed on several solid matrices

The effects of a heavy-atom salt on the room-temperature solid-matrix luminescence properties of the (+/-)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-DNA adducts [(+/-)-anti-BPDE-DNA adducts] and benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (tetrol I-1) on several solid matrices were investigated. TINO3 was found to be very effective in enhancing the room-temperature phosphorescence of the (+/-)-anti-BPDE-DNA adducts and tetrol I-1. However, the room-temperature fluorescence signals were quenched from the (+/-)-artti-BPDE-DNA adducts and tetrol I-1 on the solid matrices with the heavy-atom salt. It was found that 1% alpha-cyclodextrin/TINO3, 10% trehalose/TINO3, and 30% TINO3/sodium acetate (NaOAc) yielded very strong RTP signals from the (+/-)-anti-BPDE-DNA adducts. The greatest enhancement of the RTP intensities from the (+/-)-anti-BPDE-DNA adducts was achieved with 30% TINO3/NaOAc. In addition, a very low limit of detection (0.14 fmole/mg) for tetrol I-1 was acquired with 30% TINO3/NaOAc With its tremendous enhancement of RTP signals from the adducts and tetrol I-1 and the sub-femtomole limit of detection obtained for tetrol I-1, 30% TINO3/NaOAc is ideal for the characterization and analysis of DNA adducts and their tetrol products.