A comparison or two-dimensional electrophoresis data with phenotypical traits in Arabidopsis leads to the identification of a mutant (cri1) that accumulates cytokinins

被引：37

作者：

Santoni, V ^{[1
]}

Delarue, M ^{[1
]}

Caboche, M ^{[1
]}

Bellini, C ^{[1
]}

机构：

[1] INRA, BIOL CELLULAIRE LAB, F-78026 VERSAILLES, FRANCE

来源：

PLANTA | 1997年 / 202卷 / 01期

关键词：

Arabidopsis (mutants); biochemical classification of mutants; mutant (Arabidopsis);

D O I：

10.1007/s004250050103

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Total proteins extracted from developmental mutants of Arabidopsis thaliana (L.) Heyhn, and from wild-type plants cultivated in the presence of various hormones were analyzed by two-dimensional (2-D) gel electrophoresis. Computer analysis of 2-D gels followed by a statistical treatment of data allowed us to build a phenogram that describes the biochemical distances between the different genotypes. Analysis of the 2-D electrophoresis data allowed us to discriminate mutants in agreement with phenotypical and physiological traits. This biochemical analysis helped us to develop a working hypothesis which led us to show that one developmental mutant (cril) overaccumulates cytokinins.

引用

页码：62 / 69

页数：8