Small-molecule screening made simple for a difficult target with a signaling nucleic acid aptamer that reports on deaminase activity

被引：47

作者：

Elowe, Nadine H.

Nutiu, Razvan

Allah-Hassani, Abdellah

Cechetto, Jonathan D.

Hughes, Donald W.

Li, Yingfu

Brown, Eric A. ^{[1
]}

机构：

[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

[2] McMaster Univ, Dept Chem, Hamilton, ON L8N 3Z5, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 34期

关键词：

adenosine deaminase; assays; DNA structures; inhibitors; small-molecule screening;

D O I：

10.1002/anie.200601695

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Problem child: The application of signaling DNA-aptamer technology to the development and execution of a high-throughput screen for an otherwise problematic target, adenosine deaminase (ADA), is reported. The approach employed a signaling DNA aptamer that reports on adenosine concentration (see scheme). The assay was robust in a screen of more than 44 000 molecules and revealed a new competitive inhibitor of the deaminase. (Chemical Equation Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5648 / 5652

页数：5

共 28 条

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Aldrich MB, 2000, BIOCHEM BIOPH RES CO, V272, P311, DOI 10.1006/bbrc.2000.2773

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