Limitations of highly sensitive enzymatic presence-absence tests for detection of waterborne coliforms and Escherichia coli

被引:29
作者
VanPoucke, SO [1 ]
Nelis, HJ [1 ]
机构
[1] STATE UNIV GHENT, LAB PHARMACEUT MICROBIOL, B-9000 GHENT, BELGIUM
关键词
D O I
10.1128/AEM.63.2.771-774.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study presents evidence for the unfeasibility of enzymatic presence-absence tests to detect one total coliform or one Escherichia coli organism in 100 mi of drinking water within a working day. The results of field trials with prototype chemiluminometric procedures indicated that the sensitivity-boosting measures that are essential to achieve the required speed compromise the specificity of the tests.
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页码:771 / 774
页数:4
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