RNAi for plant functional genomics

被引：50

作者：

Matthew, L ^{[1
]}

机构：

[1] CSIRO, Platn Ind, Canberra, ACT 2601, Australia

来源：

COMPARATIVE AND FUNCTIONAL GENOMICS | 2004年 / 5卷 / 03期

关键词：

RNA interference; post-transcriptional gene silencing; high-throughput; functional genomics;

D O I：

10.1002/cfg.396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A major challenge in the post-genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out expression of a gene with the hope of seeing a phenotype that is suggestive of its function. Insertional mutagenesis is a useful tool for this type of study, but it is limited by gene redundancy, lethal knock-outs, non-tagged mutants and the inability to target the inserted element to a specific gene. RNA interference (RNAi) of plant genes, using constructs encoding self-complementary 'hairpin' RNA, largely overcomes these problems. RNAi has been used very effectively in Caenorhabditis elegans functional genomics, and resources are currently being developed for the application of RNAi to high-throughput plant functional genomics. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：240 / 244

页数：5

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