Genetic and biochemical characterization of a chromosome-encoded carbapenem-hydrolyzing Ambler class D β-lactamase from Shewanella algae

被引：69

作者：

Héritier, C ^{[1
]}

Poirel, L ^{[1
]}

Nordmann, P ^{[1
]}

机构：

[1] Univ Paris 11, Hop Bicetre, Fac Med Paris Sud, Assistance Publ Hop Paris,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 05期

关键词：

D O I：

10.1128/AAC.48.5.1670-1675.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A chromosome-encoded beta-lactamase gene from Shewanella algae clinical isolate KB-1 was cloned and expressed in Escherichia coli. It encoded the Ambler class D enzyme OXA-55, sharing less than 55% identity with any other oxacillinases. Although conferring a narrow-spectrum beta-lactam resistance phenotype, OXA-55 had carbapenem-hydrolyzing activity that mirrored the reduced susceptibility to imipenem observed in S. algae KB-1. Very similar oxacillinases were found in other S. algae isolates.

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页码：1670 / 1675

页数：6

相关论文

共 25 条

[1] Characterization of OXA-25, OXA-26, and OXA-27, molecular class D β-lactamases associated with carbapenem resistance in clinical isolates of Acinetobacter baumannii [J].

Afzal-Shah, M ;

Woodford, N ;

Livermore, DM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (02) :583-588

[2] Oxacillinase-mediated resistance to cefepime and susceptibility to ceftazidime in Pseudomonas aeruginosa [J].

Aubert, D ;

Poirel, L ;

Chevalier, J ;

Leotard, S ;

Pages, JM ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (06) :1615-1620

[3] EBR-1, a novel ambler subclass B1 β-lactamase from Empedobacter brevis [J].

Bellais, S ;

Girlich, D ;

Karim, A ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (10) :3223-3227

[4] Chromosome-encoded class D β-lactamase OXA-23 in Proteus mirabilis [J].

Bonnet, R ;

Marchandin, H ;

Chanal, C ;

Sirot, D ;

Labia, R ;

De Champs, C ;

Jumas-Bilak, E ;

Sirot, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (06) :2004-2006

[5] OXA-24, a novel class D β-lactamase with carbapenemase activity in an Acinetobacter baumannii clinical strain [J].

Bou, G ;

Oliver, A ;

Martínez-Beltran, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (06) :1556-1561

[6] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[7] PHYLOGENY OF LCR-1 AND OXA-5 WITH CLASS-A AND CLASS-D BETA-LACTAMASES [J].

COUTURE, F ;

LACHAPELLE, J ;

LEVESQUE, RC .

MOLECULAR MICROBIOLOGY, 1992, 6 (12) :1693-1705

[8] BIBI, a bioinformatics bacterial identification tool [J].

Devulder, G ;

Perrière, G ;

Baty, F ;

Flandrois, JP .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) :1785-1787

[9] Shewanella alga bacteremia in two patients with lower leg ulcers [J].

Dominguez, H ;

Vogel, BF ;

Gram, L ;

Hoffmann, S ;

Schaebel, S .

CLINICAL INFECTIOUS DISEASES, 1996, 22 (06) :1036-1039

[10] Sequence analysis of ARI-1, a novel OXA β-lactamase, responsible for imipenem resistance in Acinetobacter baumannii 6B92 [J].

Donald, HM ;

Scaife, W ;

Amyes, SGB ;

Young, HK .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (01) :196-199