Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

被引：31

作者：

Manabe, YC

Chen, JM

Ko, CG

Chen, P

Bishai, WR

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Med, Div Infect Dis,Ctr TB Res, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD USA

[3] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Ctr TB Res, Baltimore, MD USA

[4] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Int Hlth, Baltimore, MD USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 24期

关键词：

D O I：

10.1128/JB.181.24.7629-7633.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

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页码：7629 / 7633

页数：5

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