Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

被引：31

作者：

Manabe, YC

Chen, JM

Ko, CG

Chen, P

Bishai, WR

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Med, Div Infect Dis,Ctr TB Res, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD USA

[3] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Ctr TB Res, Baltimore, MD USA

[4] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Int Hlth, Baltimore, MD USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 24期

关键词：

D O I：

10.1128/JB.181.24.7629-7633.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

引用

收藏

页码：7629 / 7633

页数：5

相关论文

共 21 条

[11] Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis [J].

Manganelli, R ;

Dubnau, E ;

Tyagi, S ;

Kramer, FR ;

Smith, I .

MOLECULAR MICROBIOLOGY, 1999, 31 (02) :715-724

[12] Exposure to antibiotics induces expression of the Mycobacterium tuberculosis sigF gene:: Implications for chemotherapy against mycobacterial persistors [J].

Michele, TM ;

Ko, C ;

Bishai, WR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (02) :218-225

[13] Regulation of the inducible acetamidase gene of Mycobacterium smegmatis [J].

Parish, T ;

Mahenthiralingam, E ;

Draper, P ;

Davis, EO ;

Colston, MJ .

MICROBIOLOGY-UK, 1997, 143 :2267-2276

[14] Development and use of a conditional antisense mutagenesis system in mycobacteria [J].

Parish, T ;

Stoker, NG .

FEMS MICROBIOLOGY LETTERS, 1997, 154 (01) :151-157

[15] Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry [J].

Sonnenberg, MG ;

Belisle, JT .

INFECTION AND IMMUNITY, 1997, 65 (11) :4515-4524

[16] An inducible expression system permitting the efficient purification of a recombinant antigen from Mycobacterium smegmatis [J].

Triccas, JA ;

Parish, T ;

Britton, WJ ;

Gicquel, B .

FEMS MICROBIOLOGY LETTERS, 1998, 167 (02) :151-156

[17] 'Proteomic contigs' of Mycobacterium tuberculosis and Mycobacterium bovis (BCG) using novel immobilised pH gradients [J].

Urquhart, BL ;

Atsalos, TE ;

Roach, D ;

Basseal, DJ ;

Bjellqvist, B ;

Britton, WL ;

HumpherySmith, I .

ELECTROPHORESIS, 1997, 18 (08) :1384-1392

[18] An outbreak involving extensive transmission of a virulent strain of Mycobacterium tuberculosis [J].

Valway, SE ;

Sanchez, MPC ;

Shinnick, TF ;

Orme, I ;

Agerton, T ;

Hoy, D ;

Jones, JS ;

Westmoreland, H ;

Onorato, IM .

NEW ENGLAND JOURNAL OF MEDICINE, 1998, 338 (10) :633-639

[19] Two-dimensional electrophoresis for analysis of Mycobacterium tuberculosis culture filtrate and purification and characterization of six novel proteins [J].

Weldingh, K ;

Rosenkrands, I ;

Jacobsen, S ;

Rasmussen, PB ;

Elhay, MJ ;

Andersen, P .

INFECTION AND IMMUNITY, 1998, 66 (08) :3492-3500

[20] A mycobacterial extracytoplasmic function sigma factor involved in survival following stress [J].

Wu, QL ;

Kong, DQ ;

Lam, K ;

Husson, RN .

JOURNAL OF BACTERIOLOGY, 1997, 179 (09) :2922-2929