Diabetes alters neurite regeneration from mouse retinal explants in culture

被引：32

作者：

Takano, M

Sango, K

Horie, H

Sato, M

Iijima, Y

Ohno, S

Inoue, S

Ishikawa, Y

机构：

[1] Yokohama City Univ, Sch Med, Dept Ophthalmol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Tokyo Metropolitan Inst Neurosci, Dept Anat & Embryol, Fuchu, Tokyo 1838526, Japan

[3] Natl Inst Hlth & Nutr, Div Geriatr Hlth & Nutr, Shinjuku Ku, Tokyo 1628636, Japan

[4] Yokohama City Univ, Sch Med, Dept Physiol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

来源：

NEUROSCIENCE LETTERS | 1999年 / 275卷 / 03期

关键词：

retina; explant culture; neurite regeneration; streptozotocin-induced diabetes; mouse; adaptation to high glucose; diabetes mellitus;

D O I：

10.1016/S0304-3940(99)00768-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We examined the effect of experimental diabetes on neurite regeneration from adult mouse retinal explants cultured in the presence of different concentrations of glucose. The numbers of regenerating neu rites at 3, 6 and 10 days in culture at normal glucose concentration (7 mM) were significantly smaller in streptozotocin-induced diabetic C57BL/6 mice than in normal control mice. In contrast, treatment of retinal explants with high glucose concentration (57 mM) significantly diminished the number of regenerating neurites in the control mice, but not in the diabetic mice. These results suggest that retina in diabetic mice has impaired capability of neurite regeneration in a normal glucose environment, but is adaptable to a high glucose environment in vitro. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：175 / 178

页数：4

共 19 条

[1] Neural apoptosis in the retina during experimental and human diabetes - Early onset and effect of insulin [J].