The human cytomegalovirus ribonucleotide reductase homolog UL45 is dispensable for growth in endothelial cells, as determined by a BAC-cloned clinical isolate of human cytomegalovirus with preserved wild-type characteristics

被引：95

作者：

Hahn, G

Khan, H

Baldanti, F

Koszinowski, UH

Revello, MG

Gerna, G

机构：

[1] Univ Munich, Max Von Pettenkofer Inst Virol, D-80336 Munich, Germany

[2] Policlin San Matteo, Ist Ricovero & Cura Carattere Sci, Serv Virol, I-27100 Pavia, Italy

来源：

JOURNAL OF VIROLOGY | 2002年 / 76卷 / 18期

关键词：

D O I：

10.1128/JVI.76.18.9551-9555.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An endothelial cell-tropic and leukotropic human cytomegalovirus (HCMV) clinical isolate was cloned as a fusion-inducing factor X-bacterial artificial chromosome in Escherichia coli, and the ribonucleotide reductase homolog UL45 was deleted. Reconstituted virus RVFIX and RVDeltaUL45 grew equally well in human fibroblasts and human endothellial cells. Thus, UL45 is dispensable for growth of HCMV in both cell types.

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页码：9551 / 9555

页数：5

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