Mapping of the Physcomitrella patens proteome

被引：31

作者：

Sarnighausen, E

Wurtz, V

Heintz, D

Van Dorsselaer, A

Reski, R

机构：

[1] Univ Freiburg, D-79104 Freiburg, Germany

[2] ULP, CNRS UMR 7509, Lab Spectrometrie Masse Bioorgan, F-67087 Strasbourg 2, France

来源：

PHYTOCHEMISTRY | 2004年 / 65卷 / 11期

关键词：

Physcomitrella patens; funariaceae; moss; functional genomics; two-dimensional electrophoresis; mass spectrometry; MALDI-TOF-MS; nano LC-MS-MS;

D O I：

10.1016/j.phytochem.2004.04.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The moss Physcomitrella patens is unique among land plants due to the high rate of homologous recombination in its nuclear DNA. The feasibility of gene targeting makes Physcomitrella an unrivalled model organism in the field of plant functional genomics. To further extend the potentialities of this seed-less plant we aimed at exploring the P. patens proteome. Experimental conditions had to be adopted to meet the special requirements connected to the investigations of this moss. Here we describe the identification of 306 proteins from the protonema of Physcomitrella. Proteins were separated by two dimensional electrophoresis, excised form the gel and analysed by means of mass spectrometry. This reference map will lay the basis for further profound studies in the field of Physcomitrella proteomics. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1589 / 1607

页数：19

共 81 条

[31]

Herbert B, 2001, ELECTROPHORESIS, V22, P2046, DOI 10.1002/1522-2683(200106)22:10<2046::AID-ELPS2046>3.0.CO

[32]

2-C

[33] Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent [J].

Herbert, BR ;

Molloy, MP ;

Gooley, AA ;

Walsh, BJ ;

Bryson, WG ;

Williams, KL .

ELECTROPHORESIS, 1998, 19 (05) :845-851

[34]

Hille JM, 2001, ELECTROPHORESIS, V22, P4035, DOI 10.1002/1522-2683(200111)22:19<4035::AID-ELPS4035>3.0.CO

[35]

2-9

[36] METHODS FOR INCREASING THE RESOLUTION OF TWO-DIMENSIONAL PROTEIN ELECTROPHORESIS [J].

HOCHSTRASSER, DF ;

HARRINGTON, MG ;

HOCHSTRASSER, AC ;

MILLER, MJ ;

MERRIL, CR .

ANALYTICAL BIOCHEMISTRY, 1988, 173 (02) :424-435

[37] Optimisation of a bioreactor culture of the moss Physcomitrella patens for mass production of protoplasts [J].

Hohe, A ;

Reski, R .

PLANT SCIENCE, 2002, 163 (01) :69-74

[38] Plant functional genomics [J].

Holtorf, H ;

Guitton, MC ;

Reski, R .

NATURWISSENSCHAFTEN, 2002, 89 (06) :235-249

[39] SOLUBILIZATION OF PLANT MEMBRANE-PROTEINS FOR ANALYSIS BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS [J].

HURKMAN, WJ ;

TANAKA, CK .

PLANT PHYSIOLOGY, 1986, 81 (03) :802-806

[40]

Jacobs DI, 2001, PROTEOMICS, V1, P1345, DOI 10.1002/1615-9861(200111)1:11<1345::AID-PROT1345>3.0.CO

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