Design, synthesis and biochemical investigation, by in vitro luciferase reporter system, of peptide nucleic acids as new inhibitors of miR-509-3p involved in the regulation of cystic fibrosis diseasegene expression

被引：20

作者：

Amato, Felice ^{[1
,2
]}

Tomaiuolo, Rossella ^{[1
,2
]}

Borbone, Nicola ^{[3
]}

Elce, Ausilia ^{[1
,2
,4
]}

Amato, Jussara ^{[3
]}

D'Errico, Stefano ^{[3
]}

De Rosa, Giuseppe ^{[3
]}

Mayol, Laura ^{[3
]}

Piccialli, Gennaro ^{[3
]}

Oliviero, Giorgia ^{[3
]}

Castaldo, Giuseppe ^{[1
,2
]}

机构：

[1] Dipartimento Med Mol & Biotecnol Med, I-80131 Naples, Italy

[2] CEINGE Biotecnol Avanzate, Naples, Italy

[3] Univ Naples Federico II, Dipartimento Farm, I-80131 Naples, Italy

[4] Univ Telemat Pegaso, Naples, Italy

来源：

MEDCHEMCOMM | 2014年 / 5卷 / 01期

关键词：

TRANSMEMBRANE CONDUCTANCE REGULATOR; PNA; OLIGONUCLEOTIDES; HYBRIDIZATION; DNA;

D O I：

10.1039/c3md00257h

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Recently, microRNAs (miRNAs) were identified as being able to inhibit the expression of the Cystic Fibrosis Transmembrane Regulator (CFTR) disease-gene of Cystic Fibrosis (CF) and CFTR-Related Disorders (CFTRRD). This study shows the use of peptide nucleic acids (PNAs) as inhibitors of miR-509-3p, one of the CFTR regulating miRNAs, by reverting the expression of the luciferase gene containing the 30'UTR of CFTR gene.

引用

页码：68 / 71

页数：4

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