New calicivirus isolated from walrus

被引:17
作者
Ganova-Raeva, L
Smith, AW
Fields, H
Khudyakov, Y
机构
[1] Natl Ctr Infect Dis, Ctr Dis Control & Prevent, Div Viral Hepatitis, Atlanta, GA 30333 USA
[2] Oregon State Univ, Coll Vet Med, Lab Caliciviral Studies, Corvallis, OR 97331 USA
关键词
Vesivirus; capsid; walrus; SSH;
D O I
10.1016/j.virusres.2004.01.033
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The nucleotide sequence and genome organization of a new member of Caliciviridae was determined. Cell culture inoculated with fecal matter from walrus was used to recover fragments of a new virus by Suppression Subtractive Hybridization (SSH). The isolate was identified as a member of the Vesivirus genus of Caliciviridae and designated the name Walrus Calicivirus (WCV). Sets of PCR primers spanning the entire putative genome were designed using known sequences of other vesiviruses. The assembled genome was 8289 nucleotides (m) long and shared no more than 87% identity with sequences of the other members of the genus Vesivirus. The largest open reading frame (ORF1) between positions 4-5646 encoded a polyprotein. ORF2, found at position 5652-7778, encoded a putative capsid protein. ORF3 overlapped ORF2 and encoded a small basic protein. Comparative analysis of multiple caliciviral capsid proteins was performed to propose a uniform capsid structural organization for this viral family, (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:207 / 213
页数:7
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