Adenoviral-mediated gene expression of hepatocyte growth factor prevents postoperative peritoneal adhesion in a rat model

被引：34

作者：

Liu, Hong-Jun ^{[1
]}

Wu, Chu-Tse ^{[1
]}

Duan, Hai-Feng ^{[1
]}

Wu, Bin ^{[1
]}

Lu, Zhuo-Zhuang ^{[1
]}

Wang, Lisheng ^{[1
]}

机构：

[1] Beijing Inst Radiat Med, Dept Expt Hematol, Beijing 100850, Peoples R China

来源：

SURGERY | 2006年 / 140卷 / 03期

关键词：

D O I：

10.1016/j.surg.2005.12.014

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background. Mesothelial cell proliferation and migration play important roles in reducing formation of postoperative Periloneal adhesions. Hepatocyle growth factor (HGF) is a multifunctional cytokine that stimulates proliferation and migration of various cell types, including mesothelial cells. Methods. We investigated the effect of adenovirus-mediated HGF, gene expression on the proliferation and migration of mesothelial cells and evaluated its preventive effects on Postoperative formation of peritoneal adhesions. Rat mesothelial cells were isolated and characterized by expression of cytokeratin and vimentin. Results. Immunohistochemical staining showed that these cells expressed c-Met, the receptor for HGF. Adenoviral-mediated HGF gene transfer into mesothelial cells resulted in high expression of HGF and enhanced migration. To evaluate the preventive effects of adenoviral-mediated HGF gene transfer on the formation of postoperative peritoneal adhesion, we employed a rat model of cecum abrasion-induced adhesion formation in which 80% of the rats developed postoperative peiritoneal adhesions. Local application of recombinant adenovirus carrying the HGF gene reduced adhesion formation in 16 of 20 control rats compared with 7 of 20 treated rats in this model. Conclusions. These results suggest that adenoviral-mediated HGF gene transfer may be a novel strategy for preventing postoperative peritoneal adhesions.

引用

页码：441 / 447

页数：7

共 28 条

[21] Plasmin-induced migration of endothelial cells - A potential target for the anti-angiogenic action of angiostatin [J].

Tarui, T ;

Majumdar, M ;

Miles, LA ;

Ruf, W ;

Takada, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (37) :33564-33570

[22]

Ustun C., 2000, Journal of Obstetrics and Gynaecology (Abingdon), V20, P78

[23] Morbidity and mortality of inadvertent enterotomy during adhesiotomy [J].

van der Krabben, AA ;

Dijkstra, FR ;

Nieuwenhuijzen, M ;

Reijnen, MMPJ ;

Schaapveld, M ;

van Goor, H .

BRITISH JOURNAL OF SURGERY, 2000, 87 (04) :467-471

[24] HGF/SF induces mesothelial cell migration and proliferation by autocrine and paracrine pathways [J].

Warn, R ;

Harvey, P ;

Warn, A ;

Foley-Comer, A ;

Heldin, P ;

Versnel, M ;

Arakaki, N ;

Daikuhara, Y ;

Laurent, GJ ;

Herrick, SE ;

Mutsaers, SE .

EXPERIMENTAL CELL RESEARCH, 2001, 267 (02) :258-266

[25] Peritoneal dialysis and epithelial-to-mesenchymal transition of mesothelial cells [J].

Yañez-Mó, M ;

Lara-Pezzi, E ;

Selgas, R ;

Ramírez-Huesca, M ;

Domínguez-Jiménez, C ;

Jiménez-Heffernan, JA ;

Aguilera, A ;

Sánchez-Tomero, JA ;

Bajo, MA ;

Alvarez, V ;

Castro, MA ;

del Peso, G ;

Cirujeda, A ;

Gamallo, C ;

Sánchez-Madrid, F ;

López-Cabrera, M .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (05) :403-413

[26] Systemic administration of naked plasmid encoding hepatocyte growth factor ameliorates chronic renal fibrosis in mice [J].

Yang, J ;

Dai, C ;

Liu, Y .

GENE THERAPY, 2001, 8 (19) :1470-1479

[27]

Yao P, 2004, J HEPATOL, V40, P391, DOI 10.1016/j.jhep.2003.11.001

[28] Role of peritoneal mesothelial cells in peritonitis [J].

Yao, V ;

Platell, C ;

Hall, JC .

BRITISH JOURNAL OF SURGERY, 2003, 90 (10) :1187-1194

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