Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes

被引:264
作者
Fisher, MA
Plikaytis, BB
Shinnick, TM
机构
[1] Ctr Dis Control & Prevent, Div AIDS STD & TB Lab Res, Natl Ctr Infect Dis, Atlanta, GA 30333 USA
[2] Emory Univ, Program Microbiol & Mol Genet, Atlanta, GA 30322 USA
关键词
D O I
10.1128/JB.184.14.4025-4032.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed > 1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.
引用
收藏
页码:4025 / 4032
页数:8
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