Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis

被引：46

作者：

Busca, P

Paradisi, F

Moynihan, E

Maguire, AR ^{[1
]}

Engel, PC

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Chem, Analyt & Biol Chem Res Facil, Cork, Ireland

[2] Natl Univ Ireland Univ Coll Dublin, Dept Biochem, Conway Inst Biomol & Biomed Res, Dublin 4, Ireland

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2004年 / 2卷 / 18期

关键词：

D O I：

10.1039/b406364c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The substrate scope of three mutants of phenylalanine dehydrogenase as biocatalysts for the transformation of a series of 2-oxo acids, structurally related to phenylpyruvic acid, to the analogous alpha-amino acids, non-natural analogues of phenylalanine, has been investigated. The mutant enzymes are more tolerant than the wild type enzyme of the non-natural substrates, especially those with substituents at the 4-position on the phenyl ring. Excellent enantiocontrol resulted in all cases.

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页码：2684 / 2691

页数：8

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