Derivatization followed by reductive cleavage (DFRC method), a new method for lignin analysis: Protocol for analysis of DFRC monomers

被引：247

作者：

Lu, FC ^{[1
]}

Ralph, J ^{[1
]}

机构：

[1] UNIV WISCONSIN,USDA ARS,US DAIRY FORAGE RES CTR,DEPT FORESTRY,MADISON,WI 53706

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 07期

关键词：

acetyl bromide; lignin; beta-aryl ether; thioacidolysis; reductive elimination;

D O I：

10.1021/jf970258h

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A new method far selective and efficient cleavage of arylglycerol-beta-aryl (beta-O-4) ether linkages in lignins is introduced. The acronym ''DFRC'' relates to the reactions involved, derivatization followed by reductive cleavage. Derivatization, accompanied by cell wall solubilization, is accomplished with acetyl bromide in acetic acid; reductive cleavage of resulting beta-bromo ethers utilizes zinc in an acidic medium. Following acetylation, degradation monomers (4-acetoxycinnamyl acetates) are quantified by GC, providing data analogous to those from analytical thioacidolysis.

引用

页码：2590 / 2592

页数：3

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