Simultaneous microanalysis of N-linked oligosaccharides in a glycoprotein using microbore graphitized carbon column liquid chromatography-mass spectrometry

被引：58

作者：

Itoh, S ^{[1
]}

Kawasaki, N ^{[1
]}

Ohta, M ^{[1
]}

Hyuga, M ^{[1
]}

Hyuga, S ^{[1
]}

Hayakawa, T ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Div Biochem & Biol, Setagaya Ku, Tokyo 1588501, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 968卷 / 1-2期

关键词：

porous graphitized carbon; oligosaccharides; glycoproteins; proteins;

D O I：

10.1016/S0021-9673(02)00951-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously reported that graphitized carbon column liquid chromatography-mass spectrometry (GCC-LC-MS) is very useful for the structural analysis of carbohydrates in a glycoprotein. In this study, GCC-LC-MS was adapted for the simultaneous microanalysis of oligosaccharides. A variety of oligosaccharide alditols prepared from fetuin, ribonuclease B, and recombinant human erythropoietin were used as model oligosaccharides. The use of microbore GCC-LC-MS was found to be successful for rapid, sensitive, and simultaneous analysis of high-mannose-type, desialylated fucosyl complex-type, sialylated complex-type, and sialylated fucosyl complex-type oligosaccharide alditols. Furthermore, we demonstrate that this method is applicable to the analysis of carbohydrate heterogeneity in a glycoprotein that possesses diverse oligosaccharides. Microbore GCC-LC-MS was able to characterize high-mannose-type, hybrid-type, and complex-type oligosaccharides in tissue plasminogen activator produced from human melanoma cells in a single analysis. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：89 / 100

页数：12

共 28 条

[1] NONSELECTIVE AND EFFICIENT FLUORESCENT LABELING OF GLYCANS USING 2-AMINO BENZAMIDE AND ANTHRANILIC ACID [J].

BIGGE, JC ;

PATEL, TP ;

BRUCE, JA ;

GOULDING, PN ;

CHARLES, SM ;

PAREKH, RB .

ANALYTICAL BIOCHEMISTRY, 1995, 230 (02) :229-238

[2]

CHAN A L, 1991, Glycobiology, V1, P173, DOI 10.1093/glycob/1.2.173

[3] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF GLYCOPEPTIDES AND OLIGOSACCHARIDES ON GRAPHITIZED CARBON COLUMNS [J].

FAN, JQ ;

KONDO, A ;

KATO, I ;

LEE, YC .

ANALYTICAL BIOCHEMISTRY, 1994, 219 (02) :224-229

[4] A DETAILED STRUCTURAL CHARACTERIZATION OF RIBONUCLEASE-B OLIGOSACCHARIDES BY H-1-NMR SPECTROSCOPY AND MASS-SPECTROMETRY [J].

FU, DT ;

CHEN, L ;

ONEILL, RA .

CARBOHYDRATE RESEARCH, 1994, 261 (02) :173-186

[5]

GREEN ED, 1988, J BIOL CHEM, V263, P18253

[6] ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF PYRIDYLAMINATED OLIGOSACCHARIDE DERIVATIVES - SENSITIVITY AND IN-SOURCE FRAGMENTATION [J].

GU, JG ;

HIRAGA, T ;

WADA, Y .

BIOLOGICAL MASS SPECTROMETRY, 1994, 23 (04) :212-217

[7] CAPILLARY GEL-ELECTROPHORESIS SEPARATION OF HIGH-MANNOSE TYPE OLIGOSACCHARIDES DERIVATIZED BY 1-AMINOPYRENE-3,6,8-TRISULFONIC ACID [J].

GUTTMAN, A ;

PRITCHETT, T .

ELECTROPHORESIS, 1995, 16 (10) :1906-1911

[8] IDENTIFICATION METHOD FOR 12 OLIGOMANNOSE-TYPE SUGAR CHAINS THOUGHT TO BE PROCESSING INTERMEDIATES OF GLYCOPROTEINS [J].

HASE, S ;

NATSUKA, S ;

OKU, H ;

IKENAKA, T .

ANALYTICAL BIOCHEMISTRY, 1987, 167 (02) :321-326

[9] STRUCTURE ANALYSES OF OLIGOSACCHARIDES BY TAGGING OF REDUCING END SUGARS WITH A FLUORESCENT COMPOUND [J].

HASE, S ;

IKENAKA, T ;

MATSUSHIMA, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 85 (01) :257-263

[10] The glycosylation of Bowes melanoma tissue plasminogen activator: Lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans [J].

Jaques, AJ ;

Opdenakker, G ;

Rademacher, TW ;

Dwek, RA ;

Zamze, SE .

BIOCHEMICAL JOURNAL, 1996, 316 :427-437

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