Multiple terminal uridylyltransferases of trypanosomes

被引：25

作者：

Aphasizhev, R ^{[1
]}

Aphasizheva, I

Simpson, L

机构：

[1] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90025 USA

来源：

FEBS LETTERS | 2004年 / 572卷 / 1-3期

关键词：

Trypanosoma brucei; terminal uridylyltransferases; leishmania; RNA editing; C2H2 zinc finger;

D O I：

10.1016/j.febslet.2004.07.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transferase activities that add uridylyl residues to RNA have been reported in several unicellular and metazoan organisms. Thus far, the two terminal uridylyltransferases (TUTases) involved in uridine insertion/deletion mRNA editing in mitochondria of trypanosomes were the only known enzymes with confirmed UTP specificity. Here, we demonstrate that protein sequences of editing TUTases may be used to predict novel UTP-specific enzymes by data mining. The highest-scoring open reading frame from Trypanosoma brucei was expressed and recombinant protein purified. This enzyme catalyzes a processive UMP incorporation and is not localized to the mitochondria suggesting a non-editing biological function. (C)2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：15 / 18

页数：4

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