Characterization of five novel dehydration-responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum

被引:78
作者
Deng, X
Phillips, J
Meijer, AH
Salamini, F
Bartels, D
机构
[1] Univ Bonn, Inst Bot, D-53115 Bonn, Germany
[2] Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany
[3] Leiden Univ, Clusius Lab, Inst Mol Plant Sci, NL-2300 RA Leiden, Netherlands
[4] Genesis Res & Dev Corp Ltd, Auckland, New Zealand
基金
奥地利科学基金会;
关键词
Craterostigma plantagineum; homeodomain leucine zipper (HDZip); resurrection plant; transcription factor; yeast one-hybrid system; yeast two-hybrid interaction;
D O I
10.1023/A:1015501205303
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homeodomain leucine zipper (HDZip) genes encode putative transcription factors that are unique to plants. A function in regulating processes that are specific for plants is postulated, such as responses to environmental cues and developmental signals. This is supported by a growing body of evidence resulting from studies of HDZip genes in a variety of species. In addition to the previously isolated CPHB-1 and -2 genes, this paper reports the isolation of members of five families of Craterostigma plantagineum homeobox leucine zipper genes (CPHB) via a yeast one-hybrid screening approach. Based on the sequence homology and protein interactions the encoded proteins (CPHB-3/4/5/6/7) were classified into HDZip class II and I genes. Homo- and heterodimerization of CPHB proteins within the same structurally related class has been demonstrated and the DNA-binding activity of CPHB proteins to two homeodomain recognition elements (HDE1 and HDE2) has been compared in yeast. All families of CPHB genes were modulated in their expression in response to dehydration in leaves and roots. CPHB-6 and CPHB-7 transcripts accumulated in leaves during early stages of dehydration and decreased after prolonged dehydration. Both transcripts were also induced in ABA-treated callus. CPHB-3/4/5 were down-regulated by dehydration in both leaves and roots. The results support the role of HDZips in regulating programs of gene expression in C. plantagineum that lead to desiccation tolerance.
引用
收藏
页码:601 / 610
页数:10
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